Recombinant MHC Class II Antibody (AF405) - Primary antibody, specific to MHC Class II

Cat. No.: Ab213991
AVAILABLE TO ORDER
GRADE & PURITY 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2b
Clone Number
P24J10
Conjugation
AF405
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab213991-10μg
4
$75.90
25μg
Ab213991-25μg
3
$151.90
100μg
Ab213991-100μg
1
$377.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

0.5 mg/mL for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant MHC Class II Antibody, clone P24J10, could be used for Flow Cytometry.

Specifications

Product Name
Recombinant MHC Class II Antibody (AF405) - Primary antibody, specific to MHC Class II
Synonyms
D6S221E antibody | DMA antibody | DMB antibody | DP beta 1 antibody | DP beta 1 chain antibody | DP(W4) beta chain antibody | DPB 1 antibody | DPB1 antibody | DPB1_HUMAN antibody | DRB antibody | H2Ea antibody | HLA class II histocompatibility antigen ant
Specifications & Purity
0.5 mg/mL
Biochemical and Physiological Mechanisms
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides
Host species
Rat
Specificity
MHC Class II
Immunogen
Activated C57BL/6 mouse spleen cells
Positive Control
Flow: C57BL/6 mouse splenocytes
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Recombinant MHC Class II Antibody (AF405) (Ab213991) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant MHC Class II Antibody (AF405) (Ab213991) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Recombinant MHC Class II Antibody (AF405) (Ab213991) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant MHC Class II Antibody (AF405) (Ab213991) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
HLA-DPB1 Tbio HLA class II histocompatibility antigen, DP beta 1 chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

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