RNA Phenol Reagent - BioReagent,Suitable for molecular biology

Cat. No.: R1518313

GRADE & PURITY Suitable for molecular biology, BioReagent

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

RNA Extraction

Overview

  This reagent is an RNA extraction phenol reagent saturated with citric acid. It appears as a colorless, clear liquid with a pungent odor. The upper layer consists of a citric acid solution with pH < 5.5, while the lower layer is the organic solvent. This product does not contain 8-hydroxyquinoline and is typically used in conjunction with guanidine isothiocyanate for RNA extraction.

  This reagent is for research use only and is not intended for clinical diagnosis or other purposes.

Materials to Prepare

• Tissue Lysis Buffer

• Sodium Acetate Buffer (2M, pH 4.0)

• Chloroform

• Isoamyl alcohol

• Isopropanol

• 75% Ethanol

• TE Buffer

Procedure (For Reference Only)

Take an appropriate amount of Tissue Lysis Buffer and sequentially add the following reagents for RNA extraction:

1. Add 0.1 volume of 2 mol/L Sodium Acetate (pH 4.0) to maintain an acidic environment.

2. Add 1 volume of RNA Phenol Reagent (Note: Allow the reagent to stand before use; use the lower organic phase. The upper layer is the citric acid solution, which prevents oxidation of the lower organic phase).

3. Add 0.2 volume of Chloroform:Isoamyl alcohol (49:1) , vortex for 10 seconds to mix thoroughly, then incubate on ice for 15 minutes.

After centrifugation, carefully transfer the upper aqueous phase to a new tube. Add an equal volume of isopropanol to precipitate the RNA. Wash the precipitate once with 75% ethanol, air-dry briefly, and dissolve in 10 μL of DEPC-treated water for subsequent use.

Precautions

1. RNA Phenol Reagent is highly corrosive. Avoid skin contact or inhalation. If the reagent turns red or brown, it indicates oxidation and should not be used.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

3. Use the reagent as soon as possible after opening to prevent affecting subsequent experimental results.

Specifications

Specifications & Purity

BioReagent, Suitable for molecular biology

Stability And Storage

Store at 2-8℃ long term (12 months). Store in the dark.

Storage

Store at 2-8°C, Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Suitable for molecular biology

Chemical and Physical Properties

• Sensitivity: Light-sensitive