RNase R

Cat. No.: R749973

GRADE & PURITY Recombinant, Suitable for molecular biology, BioReagent, PCR Reagent, Endotoxin tested, Mycoplasma free, for DNA synthesis, EnzymoPure™, Endonuclease, For In Vitro Transcription, for DNA and RNA applications, Exonuclease, DNase free, ≥95%(SDS-PAGE), expressed in E.coli;20 U/μl

Accession #

P21499

Expression system

E. coli

Bioactivity

20 U/μl

Overview

Product presentation:

RNase R (Ribonuclease R) is an MG-2 + dependent exonuclease derived from the Escherichia coli RNR superfamily that completely hydrolyzes linear RNA into dinucleotides and trinucleotides from the 3'→5' direction. It is not sensitive to circular RNA and double-stranded RNA, and can be used for the production of special structural RNA, such as circRNA, lariat RNA, double-stranded RNA with 3' protruding end less than 7 nucleotides, and tRNA with complex structure. RNase R is commonly used in gene expression and variable shear studies, and can digest linear RNA to enrich circ RNA or lariat RNA.

R749973	Component	500U	2KU	10KU	Storage
R749973A	RNase R (20U/μL)	25 μL	100 μL	500 μL	-20°C. Avoid freeze/thaw cycle
R749973B	10×RNase R Buffer	500 μL	2×1 mL	10×1 mL	-20°C. Avoid freeze/thaw cycle
R749973C	RNase R Dilution Buffer	500 μL	2×1 mL	10×1 mL	-20°C. Avoid freeze/thaw cycle

Source
Recombinant E.coli
Activity unit definition
The amount of enzyme required to convert 1μg poly(A) to acid-soluble nucleotides for 10min under standard reaction conditions at 37 ° C is defined as 1U.
Recommended RNase R digestive system:

Storage buffer: 50mMTris-HCl (pH7.5 at 25℃), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1%Triton X-100 (v/v), 50%Glycerol (v/v).

Reaction Buffer: 200 mM Tris-HCl (pH 8.0 at 25°C), 1 M KCl, 1 mM MgCl₂.
Recommended RNase R digestive system:

Component	Volume
RNA	1 μg
10×RNase R Buffer	2 μL
RNase R	1-4 U/μg RNA
RNase-Free Water	Up to 20 μL

Reaction conditions: digestion at 37℃ for 15min, incubation at 70℃ for 10min can deactivate the enzyme.
* When preparing the reaction system, RNase R (20U/μL) can be diluted to a suitable working concentration by RNase R Dilution Buffer, and it is recommended to use it now.
Scope of application
Removal of linear RNA, enrichment of circ RNA from biological samples, variable shear studies, analysis and identification of intron lasso sequences, etc.
Matters needing attention
1. The play of RNase R activity requires 0.1-1.0mM Mg 2+.
2. With the increase of substrate RNA, the digestion time and enzyme amount can be appropriately prolonged.
3. The content of EDTA in RNA samples may affect the activity of RNase R. Try to reduce the amount of RNase R or shorten the digestion time.
4. The abundance of some circRNA or lasso structure RNA will decrease after prolonged digestion of RNase R, which may be due to their weak digestion ability to tolerate RNase R. Therefore, attempts can be made to reduce the amount of RNase R or shorten the digestion time.

Specifications

Product Name

RNase R

Synonyms

RNase R | Ribonuclease R

Grade

BioReagent, DNase free, Endonuclease, Endotoxin tested, Exonuclease, for DNA and RNA applications, for DNA synthesis, For In Vitro Transcription, Mycoplasma free, PCR Reagent, Recombinant, Suitable for molecular biology, EnzymoPure™

Specifications & Purity

Recombinant, BioReagent, DNase free, PCR Reagent, endotoxin tested, Mycoplasma free, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, Endonuclease, For In Vitro Transcription, for DNA and RNA applications, Exonuclease, ≥95%(SDS-PAGE), expressed in E.coli

Biochemical and Physiological Mechanisms

3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (rRNAs, tRNAs and SsrA/tmRNA). In stationary phase, involved in the post-transcriptional regulation of ompA mRNA stability. Shortens RNA proc

Bioactivity

20 U/μl

Accession #

P21499

Enzyme Commission Number

3.1.13.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli;20 U/μl

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

Under the standard reaction condition of 37℃, the amount of enzyme required to convert 1 μg of poly(A) into acid-soluble nucleotides within 10 minutes is defined as 1 unit (1 U).