Sodium citrate, also known as trisodium citrate, functions as an anticoagulant by binding to calcium ions (Ca²⁺) in the blood, forming stable soluble chelate complexes. This effectively removes the key calcium ions involved in the coagulation cascade, thereby preventing blood clotting. This product is suitable for:
Coagulation function testing: such as the determination of prothrombin time (PT), activated partial thromboplastin time (APTT), and other coagulation parameters.
Erythrocyte sedimentation rate (ESR) determination: particularly suitable for ESR measurement using the Westergren Method.
This product has undergone sterile processing.
Usage Instructions
Please ensure a thorough understanding of the experimental protocol and strictly adhere to aseptic techniques before use.
1. General Anticoagulation Ratio (Universal)
The recommended volume ratio of anticoagulant to whole blood is 1:9.
Example: Take 0.2 mL of this product, add 1.8 mL of fresh whole blood, and gently invert the tube 5-8 times to mix. Avoid vigorous shaking.
2. Special Ratio for Westergren Method ESR Determination
The recommended volume ratio of anticoagulant to whole blood is 1:4.
Example: Take 0.4 mL of this product, add 1.6 mL of fresh whole blood, and mix as described above.
Precautions
1. Intended Use: This product is for research use only. It is strictly prohibited for clinical diagnosis, blood transfusion, or human treatment.
2. Ratio Accuracy: The anticoagulant-to-blood ratio is critical. An incorrect ratio will directly affect experimental results (e.g., causing falsely prolonged or shortened coagulation times). Use precise pipetting equipment.
3. Sample Handling: Blood samples must be mixed gently immediately after collection. Avoid foaming or hemolysis.
4. Timeliness: It is recommended to complete testing of anticoagulated blood samples within 2 hours. If storage is necessary, follow the specific guidelines for your experiment (typically short-term storage at 2-8°C).
5. Personal Protection: Wear a lab coat, disposable gloves, and necessary eye protection during operation. If contact with skin or eyes occurs, rinse immediately with plenty of water.
6. Waste Disposal: All consumables that have contacted blood, residual samples, and solutions of this product must be treated as biohazardous waste, in compliance with your institution's biosafety regulations.
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