Succinimidyl PEG NHS, mPEG-NHS - MW 20000 Da

Cat. No.: S164325

GRADE & PURITY MW 20000 Da

Storage

Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Specifications

Specifications & Purity

MW 20000 Da

Biochemical and Physiological Mechanisms

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-f

Storage

Store at -20°C, Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Solubility: Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents
• Sensitivity: Moisture sensitive