UltraBio™ Antioxidant

Cat. No.: A751544

GRADE & PURITY 400X

Overview

Aladdin's UltraBio™ Antioxidant (400X) is generally used to prevent reducing proteins from being oxidized during electrophoresis with PAGE gels of neutral pH. This product is suitable for reducing protein electrophoresis with polyacrylamide gels with neutral pH, such as UltraBio™ Plus gel series or Bis-Tris precast gels.The addition of antioxidants to the running buffer in inner chamber can maintain proteins in reduced state to achieve the maximum clarity of protein bands. In gels with neutral pH, Dithiothreitol (DTT) or β-Mercaptoethanol (β-ME) in loading buffer tend to remain on top of the gel rather than migrate with proteins through the gel. Thus, some proteins may be re-oxidized during electrophoresis, which causes band diffusion. However, this antioxidant can migrate with proteins and maintain proteins in reduced state during electrophoresis, making up for the deficiency of reducing agents such as DTT.

Precautions：

This product may precipitate at low temperatures, which is normal. The precipitates can be dissolved at room temperature before use. We recommend storing this product in aliquot to avoid repeated freeze-thaw. Antioxidant (400X) is only suitable for neutral electrophoresis system, not for high pH system.Add this product to the running buffer in inner chamber only. Do not add it in the outer chamber as it will produce higher current and unnecessary waste. Running buffer with this product should not be reused.This product cannot effectively reduce disulfide bonds in proteins. We recommended using Sample Reducing Agent (10X) for protein reduction.For electrophoresis of non-reducing proteins, this product is not required.We do not recommend running reducing protein samples and non-reducing protein samples on the same gel. If it is necessary to perform such an experiment, separate reducing samples from non-reducing samples by several loading wells to avoid cross contamination. This product cannot be added into electrophoresis buffer.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Add 0.5ml of Antioxidant (400X) to the inner chamber with running buffer (cathode buffer) such as 's MES SDS Running Buffer or MOPS SDS Running Buffer , and stir appropriately.2. For detailed Western procedures, please refer to http://www.aladdin-e.com/support/western.htm.

Specifications

Product Name

UltraBio™ Antioxidant

Specifications & Purity

400X

Molecule Type

Peptide

Storage and Shipping

Concentration

400X

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Stored at -20℃ for a long time.