UltraBio™ StableLink RT-qPCR Probe Kit (UDG) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,1 μL /T

Cat. No.: FP1509102

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 1 μL /T

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, RT-qPCR

Overview

  The StableLink RT-qPCR Kit (with UDG) is a two-component, probe-based RT-qPCR master mix comprising Buffer Mix and Enzyme Mix. It is designed for sensitive fluorescent quantitative PCR detection in single or multiplex RNA targets. Optimized for performance, the buffer system is uniquely formulated to support both Taq DNA polymerase and reverse transcriptase activity, ensuring broad target compatibility and exceptional reaction stability.

  This kit incorporates a dUTP/thermolabile UDG carryover prevention system, enabling rapid degradation of uracil-containing contaminants at room temperature.

  Applications: Qualitative/quantitative PCR, multiplex PCR.

Product Component Table

FP1509102	Components	100T	1000T	10000T	Storage
FP1509102A	StableLink RT-PCR Buffer Mix	1.25 mL	10×1.25 mL	125 mL	-20℃
FP1509102B	StableLink RT-PCR Enzyme Mix	100 μL	1.0 mL	10 mL	-20℃

Protocol

Reaction Setup Preparation:

1. Thaw all required reagents at room temperature or 4°C. Mix thoroughly before use.

2. Prepare the PCR Reaction Mixture according to the table below:

Component	Volume for 25 μL System	Final Concentration
StableLink RT-PCR Buffer Mix	12.5 μL	1×
StableLink RT-PCR Enzyme Mix	1 μL	1×
Primer/Probe Mix	1 μL	/
Template	5 μL	/
ddH₂O	To 25 μL	/
Total Volume	25 μL

Note: 

"1x" indicates the working concentration of the enzyme mix as provided.

The amounts of primers, probes, and template can be adjusted according to experimental requirements.

Adjust the volumes of primers, probes, and template as needed for your specific assay; use RNase-Free ddH₂O to bring the reaction to the final volume.

To prepare reaction volumes other than 25 μL (e.g., 50 μL), scale the reagent volumes proportionally (e.g., double the volumes for a 50 μL reaction).

3. Mixing and Setup:
After preparing the reaction mixture, mix gently by pipetting or brief vortexing. Centrifuge briefly to collect the contents at the bottom of the tube. Proceed to PCR amplification.

Standard Thermal Cycling Protocol:

Step	Temperature	Time	Circulation
1	50℃	10min	1
2	95℃	2min	1
3	95℃	15s	45
4	60℃	40s (Acquire Fluorescence)	45

Specifications

Specifications & Purity

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 1 μL /T

Stability And Storage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Storage

Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology