7-Deaza-dGTP (10mM) - Animal Free, DNase, RNase, Protease free, BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, ≥99%, 10mM , CAS No.101515-08-6

CAS: 101515-08-6 Cat. No.: D759857 Molecular Weight: 506.19 EC Number: 687-837-0

GRADE & PURITY Animal Free, DNase, RNase, Protease free, Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, Sterile, ≥99%, 10mM

Synonyms

7-Deaza-dGTP

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Overview

Physical appearance: Liquid
Storage buffer solution: Ultrapure water
Product Introduction:
7-Deaza-dGTP (10mM) is a single component solution prepared with 7-Deaza-dGTP with a purity of ≥ 99%. It can effectively improve the efficiency of PCR amplification of high GC content sequences and enhance the amplification and sequencing of GC rich DNA sequences.
PCR is the most commonly used technique in many laboratories for diagnostic or molecular biology purposes, which can easily analyze large amounts of DNA sequences. However, due to the formation of secondary structures, some GC rich sequences are difficult to amplify. 7-Deaza-dGTP, also known as 7-Deaza-2 '- deoxyguanosine-5' - triphosphate, 7-deaza-2 '- deoxyguanosine-5' - triphosphate, - N7-dGTP、 7-Denitrification dGTP, etc., molecular formula C11H17N4O13P3 (Free acid), molecular weight 506.19 (Free acid), CAS Number 101515-08-6. 7-Deaza-dGTP is an artificially synthesized dGTP analog that can serve as a substrate for most DNA polymerases, including Taq DNA polymerase. Compared with dGTP, 7-Deaza-dGTP has higher chemical stability, and its incorporation into DNA can affect DNA fluorescence staining and electrophoretic mobility; Compared with dITP, 7-Deaza-dGTP improves the readability of long sequence regions. 7-Deaza-dGTP can replace or partially replace dGTP in amplifying GC rich DNA sequences, effectively reducing PCR reaction error rates, increasing PCR product yields, making DNA sequencing simpler and more efficient. It is widely used in biological experiments such as PCR amplification, sequencing, and gene cloning of GC rich DNA sequences, and plays an important role in disease diagnosis
matters needing attention:
It can dissolve at room temperature and should be stored in an ice box or on an ice bath after dissolution. After use, it should be immediately stored at -20 º C. When the PCR product contains 7-Deaza-dGTP, the reduced stacking of adjacent bases in the product makes it difficult to stain with dyes such as ethidium bromide. This product is only for scientific research by professionals and should not be used for clinical diagnosis or treatment, food or medicine, or stored in ordinary residential areas. For your safety and health, please wear lab coats and disposable gloves when operating. 

Specifications

Synonyms

7-Deaza-dGTP

Specifications & Purity

Animal Free, DNase, RNase, Protease free, BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, ≥99%, 10mM

Stability And Storage

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Storage

Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Animal Free, BioReagent, DNase, RNase free, DNase, RNase, Protease free, PCR Reagent, Sterile, Suitable for molecular biology

Action Type

INHIBITOR

Purity

≥99%

Names and Identifiers

• PH: 7.0-7.5
• Canonical Smiles: C1C(C(OC1N2C=CC3=C2N=C(NC3=O)N)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
• IUPAC Name: [[(2R,3S,5R)-5-(2-amino-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-7-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• InChIKey: DLLXAZJTLIUPAI-XLPZGREQSA-N
• INCHI: 1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)/t6-,7+,8+/m0/s1
• Isomeric SMILES: C1[C@@H]([C@H](O[C@H]1N2C=CC3=C2N=C(NC3=O)N)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
• Molecular Weight: 506.19

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Pyrrolopyrimidines
      • Subclass Pyrrolo[2,3-d]pyrimidines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Pyrrolopyrimidines
• Subclass: Pyrrolo[2,3-d]pyrimidines
• Intermediate Tree Nodes: Not available
• Direct Parent: Pyrrolo[2,3-d]pyrimidines
• Alternative Parents: Pyrimidones Monoalkyl phosphates Aminopyrimidines and derivatives Substituted pyrroles Vinylogous amides Tetrahydrofurans Heteroaromatic compounds Secondary alcohols Oxacyclic compounds Azacyclic compounds Primary amines Organopnictogen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Pyrrolo[2,3-d]pyrimidine - Monoalkyl phosphate - Pyrimidone - Aminopyrimidine - Alkyl phosphate - Substituted pyrrole - Pyrimidine - Phosphoric acid ester - Organic phosphoric acid derivative - Heteroaromatic compound - Vinylogous amide - Tetrahydrofuran - Pyrrole - Secondary alcohol - Oxacycle - Azacycle - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Primary amine - Organooxygen compound - Organonitrogen compound - Amine - Alcohol - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as pyrrolo[2,3-d]pyrimidines. These are aromatic heteropolycyclic compounds containing a pyrrolo[2,3-d]pyrimidine ring system, which is an pyrrolopyrimidine isomers having the 3 ring nitrogen atoms at the 1-, 5-, and 7-positions.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y