Amido black 10B - for electrophoresis, ≥98%(HPLC) , CAS No.1064-48-8

CAS: 1064-48-8 Cat. No.: A111676 Molecular Weight: 616.49 Beilstein Registry Number: 5374263 EC Number: 213-903-1

GRADE & PURITY Suitable for electrophoresis, ≥98%(HPLC)

Synonyms

Acid Leather Dark Blue G | Azo Dark Blue C 2B | CERN kysela 1 | Acid Black BX | Naphthol Blue Black | NIGROSINE | Sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate | Tertracid Blue Black NB | Kayaku Acid Blue Black 10

Storage

Desiccated,Room temperature

Shipped In

Normal

Overview

Naphthol Blue Black is an important and commonly used industrial dye. This dye is also used in rapid staining of protein bands on nitrocellulose membranes such as Western blots. Naphthol Blue Black can be utilized as a nucleic acid stain in SDS-Page gels. Additionally this compound has been untilized in cell proliferation assays and histochemical studies
A histological, protein, and nucleic acid dye used in electrophoresis

Specifications

Synonyms

Acid Leather Dark Blue G | Azo Dark Blue C 2B | CERN kysela 1 | Acid Black BX | Naphthol Blue Black | NIGROSINE | Sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2, 7-disulphonate | Tertracid Blue Black NB | Kayaku Acid Blue Black 10

Specifications & Purity

for electrophoresis, ≥98%(HPLC)

Storage

Desiccated, Room temperature

Shipped In

Normal

Grade

Suitable for electrophoresis

Purity

≥98%(HPLC)

Names and Identifiers

• Canonical Smiles: C1=CC=C(C=C1)N=NC2=C(C3=C(C(=C(C=C3C=C2S(=O)(=O)[O-])S(=O)(=O)[O-])N=NC4=CC=C(C=C4)[N+](=O)[O-])N)O.[Na+].[Na+]
• IUPAC Name: disodium;4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate
• InChIKey: AOMZHDJXSYHPKS-UHFFFAOYSA-L
• INCHI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2
• Isomeric SMILES: C1=CC=C(C=C1)N=NC2=C(C3=C(C(=C(C=C3C=C2S(=O)(=O)[O-])S(=O)(=O)[O-])N=NC4=CC=C(C=C4)[N+](=O)[O-])N)O.[Na+].[Na+]
• WGK Germany: 2
• RTECS: QJ6196000
• Alternate CAS: 1940-18-7
• Molecular Weight: 616.49
• Beilstein: 5374263
• Reaxy-Rn: 739194
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=739194&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Class Naphthalenes
      • Subclass Naphthalene sulfonic acids and derivatives
        • Level5 Naphthalene sulfonates
          • Level6 2-naphthalene sulfonates

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Class: Naphthalenes
• Subclass: Naphthalene sulfonic acids and derivatives
• Intermediate Tree Nodes: Naphthalene sulfonates
• Direct Parent: 2-naphthalene sulfonates
• Alternative Parents: 2-naphthalene sulfonic acids and derivatives Nitrobenzenes 1-sulfo,2-unsubstituted aromatic compounds Nitroaromatic compounds Phenoxides Sulfonyls Organosulfonic acids Azo compounds Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Primary amines Organopnictogen compounds Organooxygen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic homopolycyclic compounds
• Substituents: 2-naphthalene sulfonate - 2-naphthalene sulfonic acid or derivatives - 1-sulfo,2-unsubstituted aromatic compound - Nitrobenzene - Arylsulfonic acid or derivatives - Nitroaromatic compound - Phenoxide - Monocyclic benzene moiety - Sulfonyl - Organosulfonic acid - Organosulfonic acid or derivatives - Organic sulfonic acid or derivatives - Organic nitro compound - C-nitro compound - Azo compound - Organic alkali metal salt - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Organic oxoazanium - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organic sodium salt - Organic salt - Primary amine - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Amine - Aromatic homopolycyclic compound
• Description: This compound belongs to the class of organic compounds known as 2-naphthalene sulfonates. These are organic aromatic compounds that contain a naphthalene moiety that carries a sulfonic acid group at the 2-position. Naphthalene is a bicyclic compound that is made up of two fused benzene ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (10 mg/ml at 25 °C), and ethanol.
• Sensitivity: Hygroscopic.
• Flash Point(°F): 30 °C
• Flash Point(°C): 30°C
• Molecular Weight: 616.500 g/mol
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 14
• Rotatable Bond Count: 4
• Exact Mass: 616.006 Da
• Monoisotopic Mass: 616.006 Da
• Topological Polar Surface Area: 273.000 Ų
• Heavy Atom Count: 41
• Formal Charge: 0
• Complexity: 1110.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 3

XLogP3

References

1. Lianjie Li, Ye Jia, Ke Zeng, Zhen He, Jianqiang Xue, Anmin Liu, Tingli Ma, Liguo Gao.  (2023)  A Simple, Mild, and Low-Cost Method for Preparation of Wood-Nigrosine in Solar-Driven Interfacial Evaporation System.  Energy Technology,  11  (10): (2300531).  [PMID:] [10.1002/ente.202300531]

2. Hao Zhang, Pengfei Zhang, Zhiliang Liu, Chunfang Du.  (2023)  Polyoxometalates as highly efficient adsorbents for ciprofloxacin removal: The key role of oxygen vacancies.  JOURNAL OF MOLECULAR STRUCTURE,      [PMID:] [10.1016/j.molstruc.2023.135918]

3. Jufen Yan, Pei Wang, Longxin Wang, Qi Jin, Abdallah S. Ali, Yongsheng He, Ying Wang, Yuxuan Sun, Aoqiang Li, Waly Adwy, Rasha H. Ahmed, Xinya Han.  (2023)  Bio-decolorization of synthetic dyes by a novel endophytic fungus Penicillium janthinellum LM5 from blueberry pulp.  BIOCHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.bej.2023.108909]

4. Liuxin Li, Chunjia Luo, Xushuai Chen, Na Chu, Le Li, Min Chao, Luke Yan.  (2023)  A Novel Multifunctional Photocatalytic Separation Membrane Based on Single-Component Seaweed-Like g-C3N4.  ADVANCED FUNCTIONAL MATERIALS,  33  (23): (2213974).  [PMID:] [10.1002/adfm.202213974]

5. Jing Yang, Ligang Lin, Fengling Tang, Zixu Zhang, Zitian Liu, Meina Xu, Xu Yang, Qiying Wang, Aishan He, Qi Cheng, Chunhong Wang.  (2023)  A Bioinspired Capillary Force-Induced Driving Strategy for Constructing Ultra-Low-Pressure Separation Membranes.  ADVANCED FUNCTIONAL MATERIALS,  33  (24): (2214661).  [PMID:] [10.1002/adfm.202214661]

6. Li Xiaran, Zhu Haishan, Zhang Yuke, Meng Ruiqi, Xiong Zhong, Xia Yanzhi.  (2023)  Magnetic Alginate Carbon Aerogel Adsorbents for Water-/Oil-Soluble Dyes.  WATER AIR AND SOIL POLLUTION,  234  (3): (1-12).  [PMID:] [10.1007/s11270-023-06203-0]

7. Minghao Xu, Jiaojiao Yang, Bin Lu, Rufen Chen, Sen Wang, Hui Liu.  (2023)  Efficient degradation of ciprofloxacin by activated peroxymonosulfate using OV-rich NiCo4-LDH: Insights into radical production pathway and catalytic mechanism.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2023.141885]

8. Hukang Guo, Xiaoyi Xu, Jiaqi Li, Weilin Feng, Mengxiao Zhang, Chuanjie Fang, Liping Zhu.  (2022)  Chemically tailored microporous nanocomposite membranes with multi-channels for intensified solvent permeation.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2022.120877]

9. Lian Chen, Xianshuo Wei, Ye Sun, Yaoting Xue, Jingwen Wang, Qijun Wu, Chunxin Ma, Xuxu Yang, Gaigai Duan, Feng Wang, Shaoju Jian, Weisen Yang, Shaohua Jiang.  (2022)  A bamboo/PNIPAM composite hydrogel assembly for both programmable and remotely-controlled light-responsive biomimetic actuations.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2022.137072]

10. Amjad Hussain Memon, Bin Wei, Saira Shams, Yucui Jiang, Mengzhao Jiao, Mingming Su, Hao Liang.  (2022)  Construction of robust bienzyme-mimicking nanocatalysts for dye degradation by self-assembly of hematin, metal ions, and nucleotides.  Catalysis Science & Technology,  12  (9): (2846-2855).  [PMID:] [10.1039/D1CY01125A]

11. Weilin Feng, Jiaqi Li, Chuanjie Fang, Lin Zhang, Liping Zhu.  (2021)  Controllable thermal annealing of polyimide membranes for highly-precise organic solvent nanofiltration.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2021.120013]

12. Yan Chen, Zhao Mo, Xingtong Zhu, Qingxiang Xu, Zhaoli Xue, Henan Li, Hui Xu, Long Zhao.  (2021)  Facilitated interfacial charge separation using triphenylamine-zinc porphyrin dyad-sensitized TiO2 nanoparticles for photocatalysis.  JOURNAL OF ALLOYS AND COMPOUNDS,      [PMID:] [10.1016/j.jallcom.2021.161795]

13. Chi Zhang, Zhinan Fan, Hao Zhan, Hong Zhou, Rongliang Ma, Li-Juan Fan.  (2021)  Fluorescent Cationic Conjugated Polymer-Based Adaptive Developing Strategy for Both Sebaceous and Blood Fingerprints.  ACS Applied Materials & Interfaces,      [PMID:34080426] [10.1021/acsami.1c04741]

14. Yiling Qu, RuiYang Tao, Huan Yu, Qi Yang, Ziwei Wang, Rui Tan, Xiaochun Zhang, Ruocheng Xia, Lei Xiong, Shihan Xi, Jun Wu, Yuzhen Gao, Suhua Zhang, Chengtao Li.  (2021)  Development and validation of a forensic six-dye multiplex assay with 29 STR loci.  ELECTROPHORESIS,      [PMID:33864289] [10.1002/elps.202100019]

15. Xuerong Shui, Jiaqi Li, Mengxiao Zhang, Chuanjie Fang, Liping Zhu.  (2021)  Tailoring ultrathin microporous polyamide films with rapid solvent transport by molecular layer-by-layer deposition.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2021.119249]

16. Junfeng Chen, Hanwen Hu, Junhan Yang, Hanhan Xue, Yuping Tian, Keyan Fan, Zhixin Zeng, Jiaqi Yang, Renjun Wang, Yanyan Liu.  (2020)  Removal behaviors and mechanisms for series of azo dye wastewater by novel nano constructed macro-architectures material.  BIORESOURCE TECHNOLOGY,      [PMID:33352393] [10.1016/j.biortech.2020.124556]

17. Fan Zhinan, Zhang Chi, Ma Rongliang, Fan Li-Juan.  (2020)  Dye-soaked cotton pads for latent blood fingerprint development.  SN Applied Sciences,  2  (11): (1-14).  [PMID:] [10.1007/s42452-020-03671-5]

18. Jiaqi Li, Mengxiao Zhang, Weilin Feng, Liping Zhu, Lin Zhang.  (2020)  PIM-1 pore-filled thin film composite membranes for tunable organic solvent nanofiltration.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2020.117951]

19. Yang Xuewan, Xiong Zhong, Meng Xiao, Zhu Haishan, Xia Yanzhi.  (2020)  Broad-Spectrum Adsorption Property of Chondrus crispus Activated Carbon for Ionic and Solvent Dyes.  WATER AIR AND SOIL POLLUTION,  231  (2): (1-10).  [PMID:] [10.1007/s11270-020-4442-0]

20. Saira Shams, Waqas Ahmad, Amjad Hussain Memon, Yun Wei, Qipeng Yuan, Hao Liang.  (2019)  Facile synthesis of laccase mimic Cu/H3BTC MOF for efficient dye degradation and detection of phenolic pollutants.  RSC Advances,  9  (70): (40845-40854).  [PMID:35540072] [10.1039/C9RA07473B]

21. Tao Ruiyang, Chen Chong, Sheng Xiang, Xia Ruocheng, Zhang Xiaochun, Zhang Jingyi, Yang Zihao, Zhang Suhua, Li Chengtao.  (2019)  Validation of the Investigator 24plex QS Kit: A 6-dye Multiplex PCR Assay for Forensic Application in the Chinese Han Population.  Forensic Sciences Research,  7  (2): (172-180).  [PMID:35784410] [10.1080/20961790.2019.1665160]

22. Yuanfeng Pan, Hualei Xie, Haiyan Liu, Pingxiong Cai, Huining Xiao.  (2019)  Novel cellulose/montmorillonite mesoporous composite beads for dye removal in single and binary systems.  BIORESOURCE TECHNOLOGY,      [PMID:31030072] [10.1016/j.biortech.2019.121366]

23. Wenxia Wang, Guohe Huang, Chunjiang An, Shan Zhao, Xiujuan Chen, Peng Zhang.  (2017)  Adsorption of anionic azo dyes from aqueous solution on cationic gemini surfactant-modified flax shives: Synchrotron infrared, optimization and modeling studies.  Journal of Cleaner Production,      [PMID:] [10.1016/j.jclepro.2017.11.227]

24. Hao Liang, Shanshan Sun, Yan Zhou, Yanhui Liu.  (2017)  In-Situ Self-Assembly of Zinc/Adenine Hybrid Nanomaterials for Enzyme Immobilization.  Catalysts,  7  (11): (327).  [PMID:] [10.3390/catal7110327]

25. Yu Haili, Wang Zeru, Huang Wei.  (2017)  Highly Stable and Sensitive Colorimetric Visualization of Trivalent Chromium Using Amido Black 10B-Stabilized Silver Nanoparticles.  Plasmonics,  13  (4): (1459-1465).  [PMID:] [10.1007/s11468-017-0651-9]

26. Wan Xin Jia, Dong Tao Lu, Shao Min Shuang, Jun Yang, Chuan Dong.  (2017)  Facile Synthesis of Chitosan Modified Fe3O4 Magnetic Nanoparticles for Azo Dye Amido Black 10B Adsorption.  Journal of Nano Research,      [PMID:] [10.4028/www.scientific.net/JNanoR.49.149]

27. Jianliang Xiao, Jifei Zhang, Weiyang Lv, Yihu Song, Qiang Zheng.  (2017)  Multifunctional graphene/poly(vinyl alcohol) aerogels: In situ hydrothermal preparation and applications in broad-spectrum adsorption for dyes and oils.  CARBON,      [PMID:] [10.1016/j.carbon.2017.07.049]

28. Wenxia Wang, Guohe Huang, Chunjiang An, Xiaying Xin, Yan Zhang, Xia Liu.  (2017)  Transport behaviors of anionic azo dyes at interface between surfactant-modified flax shives and aqueous solution: Synchrotron infrared and adsorption studies.  APPLIED SURFACE SCIENCE,      [PMID:] [10.1016/j.apsusc.2017.01.311]

29. Pan Hu, Jing Wang, Ruihua Huang.  (2016)  Simultaneous removal of Cr(VI) and Amido black 10B (AB10B) from aqueous solutions using quaternized chitosan coated bentonite.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:27471086] [10.1016/j.ijbiomac.2016.07.085]

30. Jianliang Xiao, Weiyang Lv, Zhou Xie, Yeqiang Tan, Yihu Song, Qiang Zheng.  (2016)  Environmentally friendly reduced graphene oxide as a broad-spectrum adsorbent for anionic and cationic dyes via π–π interactions.  Journal of Materials Chemistry A,  4  (31): (12126-12135).  [PMID:] [10.1039/C6TA04119A]

31. Haiyan Wang, Yutong Gong, Yong Wang.  (2014)  Cellulose-based hydrophobic carbon aerogels as versatile and superior adsorbents for sewage treatment.  RSC Advances,  4  (86): (45753-45759).  [PMID:] [10.1039/C4RA08446B]

32. Si Hong, Aocheng Wei, Chao Xie, Xiaojun Shen, Jia-Long Wen, Tong-Qi Yuan.  (2024)  DES-driven sustainable dual valorization of lignocellulose into carbon dots and porous biochar for effective wastewater remediation.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39500439] [10.1016/j.ijbiomac.2024.137159]

33. Yourong Wu, Wanqian Cai, Shiqi Zhong, Cong Lin, Mei Lin, Tengfei Lin, Min Gao, Chunlin Zhao, Xiao Wu.  (2024)  Sustainable NH2-MIL-88B(Fe)/agarose carbon aerogel as a photo-Fenton catalyst for ultrafast degrading mitoxantrone.  Journal of Environmental Chemical Engineering,      [PMID:] [10.1016/j.jece.2024.113155]

34. Weilin Feng, Lu Xu, Yuji Chen, Jiaqi Li, Hukang Guo, Mengxiao Zhang, Xiaohe Wang, Jianyu Wang, Chuanjie Fang, Liping Zhu.  (2024)  Thermal-induced surface densification of polyimide asymmetrical membranes for efficient organic solvent nanofiltration.  SEPARATION AND PURIFICATION TECHNOLOGY,      [PMID:] [10.1016/j.seppur.2024.126560]

35. Fupeng Li, Jiaqi Li, Hukang Guo, Weilin Feng, Jianyu Wang, Chuanjie Fang, Liping Zhu.  (2024)  Ultramicroporous cardo-pendant polyamide nanofilms for enhanced organic solvent nanofiltration.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2024.122879]

36. Mingyuan Liu, Yue Zhang, Ying Liu, Shu Yang, Yansong Liu, Weijia Wang, Zhen Li, Wendi Liu, Liming Wang, Yanping Liu, Xiaohong Qin.  (2024)  Ultra-stable, lightweight and superelastic waste flax-based aerogel for multifunctional applications.  INDUSTRIAL CROPS AND PRODUCTS,      [PMID:] [10.1016/j.indcrop.2024.119220]

37. Xiao-Juan Wang, Kai-ting Liu, Chuan-Wan Wei, Shu-Qin Gao, Ying-Wu Lin.  (2025)  Multi-functional Fe-metallohydrogel with peroxidase/oxidase activities for dye degradation and nitrite detection.  FOOD CHEMISTRY,      [PMID:40716408] [10.1016/j.foodchem.2025.145646]

38. Yao Hui, Rukuan Liu, Jingwen Lan, Liuzemu Li, Zhihong Xiao, Airong Xu, Xuefeng Wei.  (2024)  Sodium alginate based adsorbent: Facile fabrication, extraordinary removal efficacy of anionic dyes and adsorption mechanism.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38830490] [10.1016/j.ijbiomac.2024.132842]

39. Li Haiyang, Li Xiaran, Quan Fengyu, Chen Zhaojun, Xia Yanzhi, Xiong Zhong.  (2024)  Wide-Spectrum Dye Adsorption Performance of Alginic Acid Carbon Aerogel.  WATER AIR AND SOIL POLLUTION,  235  (6): (1-12).  [PMID:] [10.1007/s11270-024-07181-7]

40. Zhenyun Wang, Zhijiao Pan, Zhongquan Zhang, Qiaohong Zhang, Kefeng Luo, Chen Chen.  (2025)  Self-template synthesis of organic-inorganic hybrid mesoporous silica and its application in efficient removal of various organic polluted species from water.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2025.138981]

41. Linkai Zhao, Jing Zhao, Zhao Mo, Ziyi Wang, Zhaoli Xue, Lei Feng, Long Zhao.  (2025)  Pyridine-substituted zinc porphyrin/TiO2 nanoparticles for increased light harvesting and efficient photocatalysis.  JOURNAL OF MOLECULAR STRUCTURE,      [PMID:] [10.1016/j.molstruc.2025.144949]

42. Hua Liu, Jinhua Zhang, Minyao Zhou, Zhaorui Liu, Junyao Qian, Jinxiang Li.  (2026)  Correlative insights into the enhanced reactivity of sulfidated zero-valent iron from different origins for the decolorization of various azo dyes under aerobic conditions.  ENVIRONMENTAL RESEARCH,      [PMID:41628723] [10.1016/j.envres.2026.123913]

43. Xiao Song, Peiqi Niu, Wenxi Gu, Chun Lam Clement Chan, Jiuhong Yi, Xu Liu, Peng Tan, Chon In Haydn Cheong, Qingwen Guan, Dan Fang, Bingpu Zhou, Zi Liang Wu, Ji Liu, Yan Yan Shery Huang, Iek Man Lei.  (2026)  Freeform Manufacturing of Plant-Based Structural Colors for Scalable Photonic and Mechanochromic Devices.  ADVANCED MATERIALS,      [PMID:41645876] [10.1002/adma.202519692]

44. Zihan Wei, Yuxin Feng, Zijing Mei, Yidong Zhang, Rukuan Liu, Sisi Liu, Airong Xu, Jin-Feng Dai.  (2026)  Boosting xanthan gum biomass adsorbent for super strong removal performances to various pollutants and mechanism.  EUROPEAN POLYMER JOURNAL,      [PMID:] [10.1016/j.eurpolymj.2026.114613]