Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Biotin-LC-Kemptide is a synthetic, biotinylated peptide substrate specifically designed for protein kinase A (PKA) activity assays and related phosphorylation research. It consists of the canonical Kemptide sequence (Leu-Arg-Arg-Ala-Ser-Leu-Gly, LRRASLG) labeled at the N-terminus with a biotin moiety linked via a 6-aminocaproic acid (LC, long chain) spacer, which minimizes steric hindrance and preserves substrate recognition by PKA.

Full Name: Biotin-LC-Leu-Arg-Arg-Ala-Ser-Leu-Gly (Biotin-LC-LRRASLG)

Molecular Formula: C₄₈H₈₆N₁₆O₁₂S (free base)

Molecular Weight: 1111.39 (free base)

Key Features & Applications

1. PKA Substrate Specificity: The central Ser residue serves as the exclusive phosphorylation site for PKA, enabling quantitative measurement of kinase activity through phosphate incorporation or antibody-based detection.

2. Biotin Affinity Tag: The biotin-LC modification allows efficient capture, purification, or immobilization of the phosphorylated peptide via streptavidin/avidin matrices, supporting high-throughput screening and pull-down assays.

3. Research Applications: Widely used in PKA activity quantification, kinase inhibitor screening, phosphorylation kinetics studies, and protein-protein interaction analysis involving PKA signaling pathways.

Specifications

Product Name

Biotin-LC-Kemptide, TFA salt

Synonyms

Grade

BioReagent

Specifications & Purity

BioReagent, ≥98%(HPLC)

Predicted molecular weight

1111.39 (free base)

Molecule Type

Peptide

Storage and Shipping

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

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Reconstitution Calculator

Reviews

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