BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Nicotinamide adenine dinucleotide (NAD) is a coenzyme present in all living cells, existing in two forms: the oxidized form (NAD⁺) and the reduced form (NADH). NAD⁺ not only functions as a coenzyme in electron transfer during redox reactions but also serves as a substrate for various enzymes in intracellular reactions. NAD⁺ plays essential roles in cells and organisms; its synthesis, degradation, and metabolic products are involved in processes such as apoptosis, metabolic regulation, and gene expression. Depletion of NAD⁺ is a major factor in cell death. The importance of NAD⁺ in regulating cellular redox status, as well as in modulating signaling pathways and transcription, has made NAD⁺ itself and its synthesis and consumption-related enzymes potential drug targets for various diseases. This kit is based on an enzymatic cycling reaction (which does not recognize NADP⁺/NADPH). In this reaction, NAD⁺ is reduced to NADH, which subsequently reduces WST-8 to form an orange-yellow formazan product with a maximum absorbance peak at approximately 450 nm. The amount of formazan generated in the reaction system is proportional to the total NAD⁺ or NADH content in the sample.
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