GRADE & PURITYfor Cell culture?Cell-culture grade — low endotoxin and contaminants to support viable cell growth. Use in mammalian/other cell culture media and supplements.100X Vitamins (Powder)
for cell culture,100X Vitamins (Powder) for Cell culture for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Desiccated Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Diamond Vitamin Tween 80 Solution 40X supplement provides nutrients and reduces the biosynthetic burden of cells in vitro. Vitamin supplements enhance the growth and prolong cell viability in cell culture media.
Application:
Diamond Vitamin Tween 80 Solution 40x has been used as a supplement in TYI-S33 medium to grow Entamoeba histolytica trophozoites.
Diamond Vitamin Tween 80 Solution 40x has been used as a supplement:
to culture Spironucleus salmonicida
to culture Giardia intestinalis
to culture Entamoeba histolytica trophozoites
Eagle’s Basal Medium (BME) was developed by Harry Eagle. BME forrmula was developed as a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. Historically, BME has been used in studies conducted to measure the growth response of normall (WI-38) and transformed (mouse and HeLa) cells in monolayer growth. It is one of the most widely used of all synthetic cell culture media.
Directions per Liter：
In method one,To prepare a 100X stock solution, dissolve 0.91g in 800-900ml of ddH2O or BME media, stirring gently with heat until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Dilute 1:100 with BME media prior to use.
Method two: 1. To prepare 10X stock solution, add 0.09g to dH2O or BME media. Dilute 1:10 with BME media. 2. To prepare 1X working solution, add 0.009g to dH2O or BME media.
Specifications
Specifications & Purity
for cell culture, 100X Vitamins (Powder)
Storage
Store at 2-8°C, Protected from light, Desiccated
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
for Cell culture
Names and Identifiers
PH
3.5-6.5
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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