Dual Color Non-denatured Gel Protein Sample Loading Buffer

Cat. No.: D752144

GRADE & PURITY 5X, Odorless

Overview

Aladdin's Dual Color Non-denaturing Protein Sample Loading Buffer (5X, Odorless) is an improved protein loading buffer with red and blue dyes, which can be used to load protein samples onto a non-denaturing PAGE gel for protein resolution.This product is safe to use. Instead of odoriferous DTT or 2-Mercaptoethanol reducing agent, this product employs an odorless and more stable reducing agent with similar reducing capability.This product has the same performance as conventional non-denaturing protein loading buffers.This product can be used for lane tracing after membrane transfer. It contains a special red dye which can be transferred to a PVDF or NC membrane to indicate the position of the lane, facilitating operations such as film cutting. Please refer to Figure 1 for the electrophoresis and the transfer results of this product.Figure 1. Aladdin's Dual Color Non-denaturing Protein Sample Loading Buffer (5X, Odorless) in the UltraBio™ Plus PAGE Precast Gel (Tris-Gly, 12%, 10 wells) after electrophoresis and transfer, as well as in the PVDF membrane after transfer (Blot).

Precautions：

This product must be completely dissolved and mixed well before use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Thaw this product at room temperature or in a water bath no more than 37℃. Place at room temperature immediately once dissolved and store at -20℃ after use.2. Add 1μl of this product per 4 μl of protein sample, and mix well. The mixture can be directly loaded to a gel.Note: After dilution to 1X, this product can also be used for sample lysis directly.3. Stop electrophoresis when the red or blue dye reaches the bottom of the gel.

Specifications

Product Name

Dual Color Non-denatured Gel Protein Sample Loading Buffer

Specifications & Purity

5X, Odorless

Molecule Type

Bioreagents/Buffer

Storage and Shipping

Concentration

5X, Odorless

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Stored at -20 ℃, valid for at least one year.