When reviewing the literature, many of them mentioned F4/80, NK1.1, CD90.1/CD90.2, CD45.1/CD45.2, which are unique to mice, are you puzzled by these unique indicators? Why do some mice use this indicator and some mice use that indicator for the same cell model? How to choose these indicators? Also, what's the deal with the FC receptor on the surface of mouse CD16/32 and how to use it? Let me answer together for you!
F4/80 - Mature Mouse Macrophage Marker
F4/80 is a 160kd glycoprotein. It is a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family.F4/80, also known as EMR1 or Ly71, is a widely used macrophage marker in mice.F4/80 is expressed in a wide variety of mature macrophages, such as koilocytes, Langerhans cells, microglial cells, and those located in the peritoneal cavity, lungs, intestines, thymus, splenic red marrow, and bone marrow stroma of Macrophages. F4/80 is a mature mouse macrophage marker and can be distinguished from other monocytes by flow assay with CD11b and F4/80.
CD16/32-Cell Surface Fc Receptor (FCR Blocker)
CD16 is a low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. They bind antibody-antigen immune complexes and mediate adaptive immune responses.CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells and dendritic cells. For the detection of mouse samples, a pure anti-mouse CD16/32 monoclonal antibody can be used as an Fc receptor blocker. In general. 100 μl volume, 10^6 cells, add 0.5-1 μg Purified Anti-Mouse CD16/32 Antibody room temperature incubation for 10 min can be added to the flow antibody, for subsequent flow cell surface or intracellular staining experiments.
Mouse NK Cell Assay for CD49b and NK1.1
For human NK cell identification, CD3-CD16+CD56+ is selected. For mouse NK cell identification, CD3-CD49b+ or CD3-NK1.1+ is selected according to the mouse strain, e.g., CD49b(DX5) for BALB/c mice and NK1.1 for C57BL/6 mice.
Differences between Mouse CD90 and CD90.1 and CD90.2
Mouse CD90 protein has two alleles, Thy1.1 (CD90.1) and Thy1.2 (CD90.2). The main difference between the two is the amino acid at position 108.CD90 is expressed on hematopoietic stem cells, neurons, all thymocytes, and peripheral T cells. Mouse strains expressing CD90.1 include AKR, BDP, MA/MyJ, etc. More mouse strains expressing CD90.2 include Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-, etc. Thy1.1 and Thy1.2 can be used in experimental studies to do cell over transfer between donors and recipients, so as to study characteristics such as the distribution of cell proliferation and differentiation.
Differences between Mouse CD45 and CD45.1 and CD45.2
Mouse CD45 protein has two alleles, CD45.1 and CD45.2. Mouse strains expressing CD45.1 include FVB, RIII, SJL/J, STS/A, DA, etc. Mouse strains expressing CD45.2 include AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, and 129. CD45.1 and CD45.2 can be used in experimental studies to do cell relay transfer between donors and recipients, so as to study the characteristics of cell proliferation and differentiation distribution.
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