γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium salt - ≥98% , CAS No.63699-78-5

CAS: 63699-78-5 Cat. No.: G265294 Molecular Weight: 328.28 EC Number: 264-418-7 PubChem CID: 16213644

GRADE & PURITY ≥98%

Synonyms

L-Glutamic acid 5-(3-carboxy-4-nitroanilide) ammonium salt | HY-15936 | EX-A4783 | L-Glutamic acid gamma-(3-carboxy-4-nitroanilide) ammonium salt, >=99.0% (TLC) | glutamic acid 5-(3-carboxy-4-nitro-anilide) ammonium S | Glupa-Carboxylate Monoammonium | H-

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Substrate for γ-glutamyltransferase

Application

Use Glupa-carboxylate in diagnostic tests for the determination of γ-glutamyltransferase, according to the recommendations of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC).

Benefits

Rely on the proven diagnostic quality of this product.

Properties

Formula: C12H12N3O7NH4

Molecular weight: 328.3 D

Specification

Appearance: White to yellowish crystalline powder

Solubility: Clear, yellow solution in water (c=100 mg/ml), tested for insolubles

pH value: 4.0-6.0

Molar rotation: [α] 25/D +32.0±2.0°

Melting range (Kofler): Approximately +170 to +180°C

Glupa-carboxylate, free acid (enzymatically): ≥87%

Glupa-carboxylate (HPLC): ≥99 area%

Water (K. Fischer): ≤6.2%

NH4 (Neßler's reagent): 5.2±1%

5-Amino-2-nitrobenzoate (HPLC): ≤0.1 area%

α-Glupa-carboxylate (HPLC): ≤0.4 area%

Thin layer chromatography (silica gel F; n-butanol/glacial acetic acid/H2O =

50/15/25; UV, with Nihydrin): Chromatographically homogeneous

A405 (Glupa-carboxylate, 6 mmol/l): 0.65-0.80

Stability: At +2 to +8°C within specification range for 24 months. Protect from light.

Specifications

Synonyms

L-Glutamic acid 5-(3-carboxy-4-nitroanilide) ammonium salt | HY-15936 | EX-A4783 | L-Glutamic acid gamma-(3-carboxy-4-nitroanilide) ammonium salt, >=99.0% (TLC) | glutamic acid 5-(3-carboxy-4-nitro-anilide) ammonium S | Glupa-Carboxylate Monoammonium | H-

Specifications & Purity

≥98%

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥98%

Names and Identifiers

• Canonical Smiles: C1=CC(=C(C=C1NC(=O)CCC(C(=O)O)N)C(=O)O)[N+](=O)[O-].N
• IUPAC Name: 5-[[(4S)-4-amino-4-carboxybutanoyl]amino]-2-nitrobenzoic acid;azane
• InChIKey: GFABNNMTRVBLPZ-QRPNPIFTSA-N
• INCHI: 1S/C12H13N3O7.H3N/c13-8(12(19)20)2-4-10(16)14-6-1-3-9(15(21)22)7(5-6)11(17)18;/h1,3,5,8H,2,4,13H2,(H,14,16)(H,17,18)(H,19,20);1H3/t8-;/m0./s1
• Isomeric SMILES: C1=CC(=C(C=C1NC(=O)CC[C@@H](C(=O)O)N)C(=O)O)[N+](=O)[O-].N
• WGK Germany: WGK 3
• PubChem CID: 16213644
• Molecular Weight: 328.28

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Glutamine and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Glutamine and derivatives
• Alternative Parents: Acylaminobenzoic acid and derivatives Nitrobenzoic acids and derivatives L-alpha-amino acids Anilides Benzoic acids Nitrobenzenes Benzoyl derivatives Nitroaromatic compounds N-arylamides Fatty amides Quaternary ammonium salts Secondary carboxylic acid amides Amino acids Carboxylic acid salts Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Monocarboxylic acids and derivatives Carboxylic acids Carbonyl compounds Organic oxides Organic salts Organic zwitterions Monoalkylamines Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Glutamine or derivatives - Acylaminobenzoic acid or derivatives - Nitrobenzoate - Alpha-amino acid - L-alpha-amino acid - Benzoic acid or derivatives - Benzoic acid - Anilide - Nitrobenzene - Nitroaromatic compound - Benzoyl - N-arylamide - Monocyclic benzene moiety - Fatty amide - Fatty acyl - Benzenoid - Quaternary ammonium salt - Secondary carboxylic acid amide - Amino acid - Carboxamide group - Carboxylic acid salt - Organic nitro compound - C-nitro compound - Allyl-type 1,3-dipolar organic compound - Propargyl-type 1,3-dipolar organic compound - Carboxylic acid - Organic oxoazanium - Organic 1,3-dipolar compound - Monocarboxylic acid or derivatives - Organic zwitterion - Primary aliphatic amine - Organic salt - Organonitrogen compound - Organooxygen compound - Primary amine - Carbonyl group - Hydrocarbon derivative - Organic nitrogen compound - Organic oxide - Organic oxygen compound - Amine - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as glutamine and derivatives. These are compounds containing glutamine or a derivative thereof resulting from reaction of glutamine at the amino group or the carboxy group, or from the replacement of any hydrogen of glycine by a heteroatom.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Clear, yellow solution in water (c=100 mg/ml)
• Molecular Weight: 328.280 g/mol
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 9
• Rotatable Bond Count: 6
• Exact Mass: 328.102 Da
• Monoisotopic Mass: 328.102 Da
• Topological Polar Surface Area: 177.000 Ų
• Heavy Atom Count: 23
• Formal Charge: 0
• Complexity: 462.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 1
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

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