Hydroxyl Magnetic Beads - DNase, RNase free, BioReagent, for DNA and RNA applications, 50mg/mL

Cat. No.: H1456630

GRADE & PURITY BioReagent, DNase, RNase free, for DNA and RNA applications, 50mg/mL

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Application

Nucleic Acid Extraction and Purification

Overview

Hydroxy magnetic beads are specially designed magnetic solid-phase carriers for nucleic acid extraction and purification. The surface silanol groups enable specific binding with nucleic acids under high-salt and low-pH conditions through hydrophobic, hydrogen bonding, and electrostatic interactions, while excluding other impurities. This allows rapid nucleic acid capture and extraction from biological samples.

With high specific surface area and magnetic susceptibility, these beads facilitate efficient nucleic acid separation. They are particularly suitable for automated liquid-phase nucleic acid extraction platforms and are widely used in biological and medical fields.

Catalog No.	Particle Size	Concentration	Recommended Applications
H1456630-APP	30-100nm	50 mg/mL	Plasmid, PCR products
H1456630-AGP	50-100nm	50 mg/mL	Genomic DNA, Plasmid
H1456630-AGV	50-100nm	50 mg/mL	Genomic DNA, Virus
H1456630-AGE	50-100nm	50 mg/mL	Genomic DNA
H1456630-AGH	200nm	50 mg/mL	Genomic DNA, Virus, Host cell residue
H1456630-BGV	200-400nm	100 mg/mL	Genomic DNA, Virus
H1456630-BGH	200-400nm	100 mg/mL	Genomic DNA, Plasmid, PCR products, Host cell residue
H1456630-BGE	200-400nm	100 mg/mL	Genomic DNA

Genomic DNA Extraction Guide

Sample Type	Recommended Catalog No.
Human whole blood	H1456630-AGE H1456630-BGE H1456630-AGP
Human swab samples	H1456630-AGE H1456630-AGV H1456630-AGH
Pig whole blood/swab samples	H1456630-BGV
Animal tissue (including FFPE)	H1456630-AGE H1456630-BGH
Bacterial genomic DNA	H1456630-AGE H1456630-AGV H1456630-BGV
Trace/degraded samples (Forensic, urine, etc.)	H1456630-AGE H1456630-AGP H1456630-AGH
Soil & feces	H1456630-AGE H1456630-BGH H1456630-AGV
Environmental samples (Soil, sewage)	H1456630-AGE H1456630-AGH H1456630-AGV
Plant tissue (high polysaccharide/phenol)	H1456630-AGE H1456630-BGH H1456630-AGV H1456630-AGH

Other Applications

Application	Recommended Catalog No.
Small/large-scale extraction	H1456630-APP H1456630-AGP H1456630-BGH
DNA/RNA viruses (Respiratory, reproductive, animal, other infections)	H1456630-AGV H1456630-AGH H1456630-BGV
PCR product purification	H1456630-APP H1456630-BGH
Host cell residual nucleic acids	H1456630-BGH H1456630-AGH

Key Features

1. Excellent handling performance

2. High specific surface area with strong binding capacity

3. High reproducibility

Protocol

1. Prepare lysed or dissolved sample solution.

2. Add appropriate volume of magnetic beads and incubate.

3. Separate bead-bound nucleic acids from unbound materials using magnetic separation.

4. Wash beads with wash buffer to remove impurities; separate using magnets.

5. Elute nucleic acids with elution buffer; separate beads from product magnetically.

Note: Extraction steps and buffer systems require optimization for specific sample types and targets (virus/bacteria/gDNA/RNA, etc.). 

Precautions

1. Storage:

   • 1 year at room temperature; 2 years at 2-8°C.

   • Slight color variations under normal storage conditions do not affect performance.

2. Handling:

   • Avoid freezing, drying, or centrifugation (may cause bead aggregation and resuspension issues).

3. Usage:

   • Vortex thoroughly before use to ensure uniform suspension.

Specifications

Specifications & Purity

DNase, RNase free, BioReagent, for DNA and RNA applications, 50mg/mL

Stability And Storage

Store at 2-8℃ long term (24 months). Do not freeze.

Storage

Store at 2-8°C, Do not freeze

Shipped In

Wet ice, Do not freeze

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications