GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥98%(SDS-PAGE)1.0 mg/mL
Accession #
P08659
Protein Tag
N-His
Expression system
E. coli
Bioactivity
>10^9 RLU/mg protein as determined in the presence of ATP and luciferin.
Recombinant,EnzymoPure™,His-Tag,≥98%(SDS-PAGE),1.0 mg/mL His Tag,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Firefly Luciferase catalyzes the reaction of luciferin with ATP and results in the production of yellow-green light. The enzyme has a molecular weight of 62 kDa and is expressed in peroxisomes. Luciferase is considered a model for studying protein-anesthetic interactions. Firefly Luciferaseis very useful in cell biology and molecular biology as a reporter of gene function and for the quantification of ATP.
Sequence: Amino acid sequence of luciferase derived fromPhotinus pyralis(firefly) was expressed with 6×His tag at the N-terminus.
Protein length: The recombinant Firefly Luciferase predicts a molecular mass of 62kDa.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
1.Wang Zhengwei, Lin Jiani, Tan Guisen, Pan Tingzheng, Zhang Hongtian, Liu Jiangang. (2025) miR-1270 suppresses glioblastoma development by transcriptional inhibition of nuclear factor IX. MOLECULAR BIOLOGY REPORTS, 52 (1):(1-10). [PMID:40815328][10.1007/s11033-025-10915-3]
2.Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo. (2025) Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation. PLANT CELL AND ENVIRONMENT, [PMID:40947544][10.1111/pce.70180]
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