GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5mg/mL
ExactAb™, Validated, 0.5mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse Anti-Human IgG Antibody (mAb), Secondary antibody, could be used for IF/ICC and Flow Cytometry.
Specifications
Product Name
Mouse Anti-Human IgG (FITC) - Secondary antibody, specific to Human IgG
Synonyms
Mouse Anti-Human IgG Antibody | Mouse Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody | FLJ39988 antibodyFLJ40036 antibody | FLJ40253 antibody | FLJ40587 antibody | FLJ40789 antibody | FLJ40834 antibody | G
Specifications & Purity
ExactAb™, Validated, 0.5mg/mL
Host species
Mouse
Specificity
Human IgG
Immunogen
Human IgG
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Octylic acid-ammonium sulfate precipitation
Concentration
0.5mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Mouse Anti-Human IgG (FITC) (Ab226748) - IF/ICC IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Mouse Anti-Human IgG (FITC) (Ab226748) at a dilution of 1/500 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Mouse Anti-Human IgG (FITC) (Ab226748) - Flow Cytometry Two-color flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (APC) (Ab224677) and either Mouse IgG (FITC) (Ab180307; Left panel) or Mouse Anti-Human IgG (FITC) (Ab226748; Right panel) at 0.3μg/test.
Mouse Anti-Human IgG (FITC) (Ab226748) - Flow Cytometry Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Mouse Anti-Human IgG (FITC) (Ab226748) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
IF/ICC
1:500
Flow Cytometry
1/50 - 1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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