Neutral Protease from Bacillus polymyxa(Partially Purified), CAS No.9001-92-7

CAS: 9001-92-7 Cat. No.: N128694 EC Number: 232-642-4
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GRADE & PURITY EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. ≥0.1 units/mg dry weight
Accession #
P07518
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Status
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250mg
N128694-250mg
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1g
N128694-1g
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Why this grade

EnzymoPure™, ≥0.1 units/mg dry weight EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 28 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Neutral protease (Dispase&reg) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Aladdin. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.

Specifications

Product Name
Neutral Protease from Bacillus polymyxa(Partially Purified), CAS No.9001-92-7
Synonyms
Dispase | Proteinase | Bacillus polymyxa neutral
Grade
EnzymoPure™
Specifications & Purity
EnzymoPure™, ≥0.1 units/mg dry weight
Accession #
CAS
9001-92-7
Enzyme Commission Number
3.4.24.28
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥0.1 units/mg dry weight
Storage
Store at 2-8°C
Shipped In
Wet ice
Unit definition
One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

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🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

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Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
J22181197Certificate of AnalysisOct 29, 2025 N128694
L1805093Certificate of AnalysisMay 06, 2022 N128694
Documents & Articles
Advanced enzyme analysis technology
Protein sample preparation process
Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes
Principles and Selection Strategies for Common Tissue Dissociation Enzymes
Recent Advances in Methods for Measuring Plant Fiber-Related Indices and Application-Oriented Selection
Methodological Studies on Glycoprotein Structural Characterization and Quality Attribute Analysis
The uPA/uPAR System in Cell Migration and Microenvironment Remodeling
Application of Fatty Acid Methyl Ester Sulfonate (MES) in Household Detergent and Cleaning Formulations: Detergency, Hard-Water Tolerance, and Selection Criteria
Citations of This Product
References
1. Run Ji, Xiuling Zhang, Chenghai Liu, Wentao Zhang, Xiaofeng Han, Hengtian Zhao.  (2023)  Effects of extraction methods on the structure and functional properties of soluble dietary fiber from blue honeysuckle (Lonicera caerulea L.) berry.  FOOD CHEMISTRY,      [PMID:37591145] [10.1016/j.foodchem.2023.137135]
2. Ming Tian, Chen Ning, Siyuan Peng, Deyu Li, Renyi Jin, Yang Zhang, Zhemin Liu, Haijin Mou, Changliang Zhu.  (2023)  High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate.  Foods,  12  (6): (1252).  [PMID:36981178] [10.3390/foods12061252]
3. Yuzhe Huang, Kunfeng Zhang, Wenqiang Guo, Chan Zhang, Hao Chen, Tenglong Xu, Yongming Lu, Qingxi Wu, Yong Li, Yan Chen.  (2022)  Aspergillus niger fermented Tartary buckwheat ameliorates obesity and gut microbiota dysbiosis through the NLRP3/Caspase-1 signaling pathway in high-fat diet mice.  Journal of Functional Foods,      [PMID:] [10.1016/j.jff.2022.105171]
4. Ji-Xin Li, Shu-Xiang Zhao, Yu-Qing Zhang.  (2022)  Silk Protein Composite Bioinks and Their 3D Scaffolds and In Vitro Characterization.  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,  23  (2): (910).  [PMID:35055092] [10.3390/ijms23020910]
5. Hai-Yan Wang, Su-Fang Zhou, Meng Zhang, Heng-Da Wang, Yu-Qing Zhang.  (2021)  The post-processing temperature or humidity can importantly control the secondary structure and characteristics of silk fibroin films.  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,  110  (4): (827-837).  [PMID:34766716] [10.1002/jbm.a.37331]
6. Hongxia Tan, Hongyuan Zhou, Ting Guo, Yuhao Zhang, Liang Ma.  (2021)  Zein-bound zearalenone: A hidden mycotoxin found in maize and maize-products.  FOOD CONTROL,      [PMID:] [10.1016/j.foodcont.2021.107903]
7. Jiaxi Chu, Haizhen Zhao, Zhaoxin Lu, Fengxia Lu, Xiaomei Bie, Chong Zhang.  (2019)  Improved physicochemical and functional properties of dietary fiber from millet bran fermented by Bacillus natto.  FOOD CHEMISTRY,      [PMID:31126508] [10.1016/j.foodchem.2019.05.035]
8. Jian-Yuan Yang, Jing Li, Mei Wang, Xian-Guo Zou, Bin Peng, Yu-Long Yin, Ze-Yuan Deng.  (2019)  A Novel Aqueous Extraction for Camellia Oil by Emulsified Oil: A Frozen/Thawed Method.  EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,  121  (4): (1800431).  [PMID:] [10.1002/ejlt.201800431]
9. Yu Zhu, Chenghu He, Haixiao Fan, Zhaoxin Lu, Fengxia Lu, Haizhen Zhao.  (2018)  Modification of foxtail millet (Setaria italica) bran dietary fiber by xylanase-catalyzed hydrolysis improves its cholesterol-binding capacity.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2018.11.052]
10. Yu Zhu, Jiaxi Chu, Zhaoxin Lu, Fengxia Lv, Xiaomei Bie, Chong Zhang, Haizhen Zhao.  (2017)  Physicochemical and functional properties of dietary fiber from foxtail millet (Setaria italic) bran.  JOURNAL OF CEREAL SCIENCE,      [PMID:] [10.1016/j.jcs.2017.12.011]
11. Chang-Wei Zhang, Cheng-Zhang Wang, Ran Tao, Chuanjie Fang.  (2015)  Enzymolysis-based ultrasound extraction and antioxidant activities of polyprenol lipids from Ginkgo biloba leaves.  PROCESS BIOCHEMISTRY,      [PMID:] [10.1016/j.procbio.2015.12.008]
12. Zong-Bo Xie, Da-Zhao Sun, Guo-Fang Jiang, Zhang-Gao Le.  (2014)  Facile Synthesis of Bis(indolyl)methanes Catalyzed by α-Chymotrypsin.  MOLECULES,  19  (12): (19665-19677).  [PMID:25438078] [10.3390/molecules191219665]
13. Xiang Zou, Junguo He, Xinlei Pan, Qiupeng Cai, Shengye Duan, Yijie Zhong, Xinxin Cui, Jie Zhang.  (2024)  Investigating enhancement of protease and lysozyme combination pretreatment on hydrolysis of sludge organics under humic acid inhibition.  BIORESOURCE TECHNOLOGY,      [PMID:39643052] [10.1016/j.biortech.2024.131928]
14. Yanbei Zhang, Jie Li, Shoulei Yan.  (2025)  Mechanism study on removal of browning pigment from high temperature sterilized lotus rhizome juice by lactic acid bacteria fermentation.  FOOD CHEMISTRY,      [PMID:39889642] [10.1016/j.foodchem.2025.143073]
15. Jiaming Cao, Boyuan Xiang, Baojie Dou, Jingfei Hu, Lei Zhang, Xinxin Kang, Mingsheng Lyu, Shujun Wang.  (2024)  Novel Angiotensin-Converting Enzyme-Inhibitory Peptides Obtained from Trichiurus lepturus: Preparation, Identification and Potential Antihypertensive Mechanism.  Biomolecules,  14  (5): (581).  [PMID:38785988] [10.3390/biom14050581]
16. Fan-Yu He, Xing-Tong Zhu, Hui Liu, Yun-Q ing Chong, Zhi-Ping Wu, Lu-Jun Ye, Yue-Wen Chen, Jing-Jing Fu.  (2024)  Structural and sensory characteristics of ultrasonic assisted wet-heating Maillard reaction products of Giant salamander protein hydrolysates.  JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,      [PMID:39056251] [10.1002/jsfa.13769]
17. Zhenhao Zhao, Junbo Hu, Huimin Cui, Tianrui Zhao, Yaping Liu, Zhengxuan Wang, Guiguang Cheng.  (2025)  Tyrosinase inhibition and enzyme kinetics red rice bran protein peptides: mechanism, identification, in silico exploration, and experimental validation.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.106319]
18. Dongkun Tu, Ye Xu, Yuxin Cheng, Yuhua Huang, Yingting Zhao, Yuting Tian.  (2024)  Ultrasound-assisted microwave vacuum drying enhances flavor release during stewing of dried shiitake mushrooms.  FOOD CHEMISTRY,      [PMID:39577260] [10.1016/j.foodchem.2024.142014]
19. Jing Zhao, Shibo Cheng, Xin Wang, Ruilian Yu, Bin Du, Jiajun Tong, Yiwei Liu, Taiping Qing, Xiaofeng Liu.  (2025)  Urease-mediated homogeneous dual-mode biosensor for on-site qualitative and accurate sensitive quantitative detection of toxic soluble Aβ oligomers in undiluted samples.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.166632]
20. Suxin Li, Jinhui Gu, Yiyi Liu, Weiqiang Qiu, Wenzheng Shi.  (2025)  Physicochemical Properties and Stability of Antioxidant Peptides from Swim Bladder of Grass Carp (Ctenopharyngodon idella).  Foods,  14  (7): (1216).  [PMID:40238430] [10.3390/foods14071216]
21. Haibin Yuan, Tianyang Wang, Lan Xiao, Hongfeng Jia, Huachang Wu, Ping Dong.  (2025)  Comprehensive Analysis of Volatile and Nonvolatile Compounds in Dictyophora indusiata Hydrolysates.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2025.117737]
22. Wu Qiong, Wu Xinru, Wang Zifei, Li Zhentao, Wang Jiaming, Liang Bin, Dai Yonggang.  (2025)  Effect of fermentation on the structure and properties of soluble dietary fiber from peanut shells.  Journal of Food Measurement and Characterization,      [PMID:] [10.1007/s11694-025-03294-7]
23. Qiong Wu, Xinru Wu, Zifei Wang, Zhitong Cai, Yonggang Dai.  (2025)  Fermentation-synergized physical modification: Enhancing physicochemical properties and bioactivities of soluble dietary fiber from peanut shells.  Food Chemistry-X,      [PMID:40606749] [10.1016/j.fochx.2025.102667]
24. Yumei Feng, Yuanmin Zhu, Bohan Wang, Feifan Zhao, Yanxia Zhang, Xinqi Zhu, Huaixin Li, Longjiang Yu.  (2025)  Enhanced docosahexaenoic acid production of Schizochytrium sp. H016 utilizing rapeseed meal hydrolysate.  PROCESS BIOCHEMISTRY,      [PMID:] [10.1016/j.procbio.2025.10.011]
25. Jun-Hui Du, Yu-Yun Xiao, Ying-Chao Guo, Wen-Qing Zhang, Guan-Ru Si, Zheng-Fang Wu, Chuan-Qiang Gao, Jie Mei, Yi-Long Ma, Zhao-Jun Wei.  (2025)  Identification and Inhibitory Mechanisms of Anti-α-glucosidase Peptides from Xiaojiao Baijiu Distillers’ Grains.  ACS Food Science & Technology,      [PMID:] [10.1021/acsfoodscitech.5c00605]
26. Long Chen, Huahui Ren, Liang Li, Jihao Zuo, Jinting Cai, Ying Li, Huayao Chen, Li Hao, Hongjun Zhou, Zhanghua Zeng, Xinhua Zhou.  (2025)  Enzymatically degradable pesticide microcapsules fabricated from zein nanoparticle-stabilized Pickering emulsions for enhanced safety and prolonged crop protection.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.170947]
27. Yuxi Guo, Mengmeng Xu, Zhongbao Ma, Zhenghua Gu, Lei Chen, Guiyang Shi, Zhongyang Ding.  (2025)  Amino-functionalized magnetic beads for green and selective deproteinization of polysaccharides from edible and medicinal fungi.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:41276035] [10.1016/j.ijbiomac.2025.149200]
28. Xinru Wu, Yonggang Dai, Zhitong Cai, Jiaxiu Wei, Qiong Wu.  (2025)  Structural and functional optimization of fermentation-mediated peanut shells insoluble dietary fiber and its regulatory effect on cookies quality.  FOOD CHEMISTRY,      [PMID:41418544] [10.1016/j.foodchem.2025.147582]
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