NeutrAvidin Protein

Cat. No.: N638951

Bioactivity

11-17 μg biotin bound/mg protein.

Overview

Product introduction：
NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding.
Features include:
• Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin
• Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin
• No RYD recognition sequence—no known off-target binding domains like streptavidin
• Affordable—significantly less expensive than streptavidin
NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.

Specifications

Product Name

NeutrAvidin Protein

Synonyms

Nuetral Avidin | NeutrAvidin

Bioactivity

11-17 μg biotin bound/mg protein.

Conjugation

Unconjugated

Predicted molecular weight

60 kDa

Molecule Type

Protein

Storage and Shipping

Shape

Solid

Reconstitution

Reconstitute NeutrAvidin Protein with ultrapure water and then dilute to 1 mg/mL with PBS or other buffer. NeutrAvidin Protein is not directly soluble in phosphate-buffered saline (PBS). NeutrAvidin Protein can precipitate in solutions close to its isoelectric point (6.3 ± 0.3). Solutions containing 10% glycerol may aid initial solubilization and prevent precipitation. Glycerol does not interfere with NeutrAvidin Protein’s ability to bind biotin.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (1 year). Upon reconstitution, it is recommended to aliquot.