GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
Recombinant,ExactAb™,Validated,5 μL/test ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Recombinant CD19 Antibody, clone P03D32, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD19 Antibody (PE-Cy7) - Primary antibody, specific to CD19
Synonyms
Antibody deficiency due to defect in CD19 antibody | Antibody deficiency due to defect in CD19, included antibody | AW495831 antibody | B lymphocyte antigen CD19 antibody | B lymphocyte surface antigen B4 antibody | B-lymphocyte antigen CD19 antibody | B-
Specifications & Purity
Recombinant, ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808).
Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16
Host species
Mouse
Specificity
CD19
Positive Control
Flow Cytometry: Human peripheral blood lymphocyte
Conjugation
PE-Cy7
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
774nm
Ex/Em(nm)
Ex:565nm;Em:774nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (PE-Cy7) (Ab229556) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy7) (Ab190079).
Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (PE-Cy7) (Ab229556) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy7) (Ab190079). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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