GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P29320
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
1、Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA3 is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 ‑ 25 ng/mL. 2、Immobilized Recombinant Human EphA3 Protein (rp145609) at 1.0 μg/mL can bind Ifabotuzumab (anti-EphA3) (Ab191893) with the EC50 is 13.82 ng/mL.
1、Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA3 is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 ‑ 25 ng/mL. 2、Immobilized Recombinant Human EphA3 Protein (rp145609) at 1.0 μg/mL can bind Ifabotuzumab (anti-EphA3) (Ab191893) with the EC50 is 13.82 ng/mL.
65 - 80 kDa, under reducing conditions; 60 - 75 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Shape
Liquid
Reconstitution
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human EphA3 Protein (rp145609) - Protein Bioactivity Immobilized Recombinant Human EphA3 Protein (rp145609) at 1.0 μg/mL can bind Ifabotuzumab (anti-EphA3) (Ab191893) with the EC50 is 13.82 ng/mL.
Recombinant Human EphA3 Protein (rp145609) - SDS-PAGE 2 μg/lane of Recombinant Human EphA3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 65 - 80 kDa under reducing conditions and 60 - 75 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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