GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.≥95%(SDS-PAGE)See COA
Accession #
Q15465
Protein Tag
No tag
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human Sonic Hedgehog Protein (rp228951) at 1.0 μg/mL can bind Sonic Hedgehog Mouse mAb (Ab128686) with the EC50 of 20.8 ng/mL.
Animal Free, Carrier Free, Bioactive, ActiBioPure™, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the g
Bioactivity
Immobilized Recombinant Human Sonic Hedgehog Protein (rp228951) at 1.0 μg/mL can bind Sonic Hedgehog Mouse mAb (Ab128686) with the EC50 of 20.8 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human Sonic Hedgehog Protein (rp228951) - ELISA
Immobilized Recombinant Human Sonic Hedgehog Protein (rp228951) at 1.0 μg/mL can bind Sonic Hedgehog Mouse mAb (Ab128686) with the EC50 of 20.8 ng/mL.
Recombinant Human Sonic Hedgehog Protein (rp228951) - SDS-PAGE
3 μg/lane of Recombinant Human Sonic Hedgehog Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 19.4 & 32.8-44.6 kDa under reducing conditions and 19.5 & 31.6-44.9 & 54.5-63.6 & 75.2-86.8 kDa under non-reducing conditions.
Sonic Hedgehog can form Multimer. Multiple bands correspond to multiple conformations, which caused by incomplete autocleavage in animal-component-free medium (Uniprot: Q15465).
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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