Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566)

Cat. No.: rp220557

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Expression system

E. coli

Bioactivity

Binding Biotin

Overview

Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.

Specifications

Product Name

Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566)

Synonyms

SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin

Grade

ExactAb™, Validated

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Bioactivity

Binding Biotin

Conjugation

LumiDye™ Yellow-Green 566

Excitation (nm)

Green Laser(532nm)

Emission(nm)

566nm

Ex/Em(nm)

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Images

IF/ICC: Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566) (rp220557)

Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566) (rp220557) – IF/ICC
IF analysis of GAPDH (red) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Biotin) (Ab219252) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566) (rp220557) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566) (rp220557)

Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566) (rp220557) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213118) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 566) (rp220557) at 1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  1-10μg/mL;

• Flow Cytometry:

  1.0 -10μg/mL; Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.;