UltraBio™ StableMax RT-qPCR Master Mix (Dye, UDG, One Tube) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,10 μL/T

Cat. No.: FP1508907

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 10 μL/T

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, RT-qPCR

Overview

  The StableMax RT-qPCR Master Mix (with Dye, UDG) is a one-tube, probe-based RT-qPCR premix optimized for both single and multiplex RNA detection. Its unique buffer system is compatible with Taq DNA polymerase and reverse transcriptase, enabling seamless reverse transcription and amplification in a single reaction.

Catalog	Component	100T	1000T	10000T	Storage
U1492577	UltraBio™ StableMax RT-qPCR Master Mix (Dye, UDG, One Tube)	1.0 mL	10×1.0 mL	100mL	-20℃

Key Features:
✔ Visual tracking: Yellow dye (non-inhibitory) confirms reagent addition, preventing omissions
✔ Contamination control: dUTP/UDG system eliminates carryover amplicon contamination
✔ Broad applications: Suitable for one-step RT-qPCR (RNA targets) and hot-start DNA amplification
✔ Optimized performance: High sensitivity and reproducibility for low-abundance targets

Protocol

I. Reaction Setup

1. Reagent Preparation

   • Thaw all components at room temperature or 4°C.

   • Mix gently and centrifuge briefly before use.

   • Recommendations:

     • Protect probes from light.

     • Aliquot master mix to avoid freeze-thaw cycles.

2. Prepare Reaction Mix

Component	Volume per 25 μL	Volume per 50 μL	Final Concentration
StableMax RT-PCR Master Mix (Dye, UDG, One Tube)	10 μL	20 μL	1×
Primer-probe Mix	1 μL	2μL	/
Templates	5 μL	10 μL	/
ddH₂O	Up to 25 μL	Up to 50 μL	/
Total	25 μL	50 μL	/

*Note:

• Primer/template volumes can be adjusted.

• For multiplex assays, optimize primer concentrations empirically.

1. Mixing:

   • Gently pipette mix or vortex briefly.

   • Centrifuge at room temperature to collect liquid.

2. PCR Amplification:

   • Load tubes onto a thermal cycler with heated lid (105°C recommended).

II. Thermal Cycling Program

Step	Temperature	Time	Cycles
1	50℃	15min	1
2	95℃	2min 30s	1
3	94℃	10s	45
4	55℃	40s（Fluorescence read）	45

Optimization Guidelines:
a. Reverse Transcription: 5–15 min (adjust based on target abundance).
b. Cycle Number: Reduce to 40 cycles for high-copy targets.
c. Primer Design:

• Final concentration: 0.2 μM (typical).

• Tm ≥55°C (use software for accurate calculation).

• Adjust annealing temperature (±3°C) if amplification is suboptimal.

Specifications

Specifications & Purity

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 10 μL/T

Stability And Storage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Storage

Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology