UltraBio™ StableMax RT-qPCR Master Mix (UDG, One Tube) - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,10 μL/T

Cat. No.: FP1508908

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 10 μL/T

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR, RT-qPCR

Overview

  StableMax RT-qPCR Master Mix (with UDG) is a ready-to-use, all-in-one probe-based master mix for single/multiplex RT-qPCR detection of RNA targets. Optimized buffer simultaneously supports Taq DNA polymerase and reverse transcriptase activity, ensuring broad target compatibility. Pre-mixed working solutions containing primers/probes remain stable at low temperatures without compromising performance. Incorporates dUTP/UDG contamination control to eliminate carryover amplicon contamination. Compatible with standard and fast cycling protocols.

Component

Catalog	Component	100T	1000T	10000T	Storage
FP1508908	UltraBio™ StableMax RT-qPCR Master Mix (UDG, One Tube)	1.0 mL	10×1.0 mL	100mL	-20℃

  Applications:

• One-step RT-qPCR (RNA targets)

• Hot-start DNA amplification

Key Advantages

1. Unified Buffer System: Optimized for both Taq polymerase and reverse transcriptase.

2. Ready-to-Detect: Add primers/probes to master mix → store as working solution → add sample for direct use.

3. Contamination Control: dUTP/UDG system cleaves uracil-containing contaminants.

4. Broad Compatibility: Adapts to fast cycling (≤40 min) and multiplex assays.

Protocol

1. Reaction Setup

Component	Volume/25 μL	Volume/50 μL	Final Conc.
StableMax RT-qPCR Master Mix	10 μL	20 μL	1×
Primer-probe Mix	1 μL	2μL	/
Templates	5 μL	10 μL	/
ddH₂O	Up to 25 μL	Up to 50 μL	/
Total	25 μL	50 μL	/

Notes:

• Primer/template volumes are adjustable.

• For fast cycling, use 25 μL total volume.

• Protect probes from light; avoid freeze-thaw cycles.

2. Mixing & Centrifugation

• Gently pipette-mix or vortex lightly.

• Centrifuge briefly to collect liquid.

3. Cycling Conditions

Step	Temperature	时间	循环数
1	50℃	15min	1
2	95℃	2min 30s	1
3	94℃	10s	45
4	55℃	40s（collect fluorescence）	45

Notes

1. Reverse transcription (adjust: 5–15 min)
2. Initial denaturation (adjust: 1.5–2.5 min)
3. Reduce to 40 cycles for high-copy targets.
4. Primer Design:
Final concentration: 0.2 μM
Tm ≥55°C (design with tools like OligoAnalyzer™).

Specifications

Specifications & Purity

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 10 μL/T

Stability And Storage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Storage

Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology