Zymolyase, Arthrobacter luteus, CAS No.37340-57-1

CAS: 37340-57-1 Cat. No.: Z1421592 EC Number: 609-409-4

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Arthrobacter luteus; ≥20000 U/g enzyme powder

Bioactivity

≥20000 U/g enzyme powder

Overview

Zymolyase, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.

Product Information

Form	Lyophilized Powder
Purification	Ammonium Sulfate Precipitation
Activity	20,000 units/g
Essential enzyme	β-1,3-glucan laminaripentaohydrolase
Other activities contained (※1)	β-1,3-glucanase:  approx. 1.5 × 10⁶ units/g Protease: approx. 1.0 × 10⁴ units/g Mannanase: approx. 1.0 × 10⁶ units/g
Contaminants (Amylase, Xylanase, Phosphatase)	Trace amount
Optimum pH and Temp.	pH 7.5, 35°C (for lysis of viable yeast cells) pH 6.5, 45°C (for hydrolysis of yeast glucan)
Stability (2°C)	No loss of activity was found after storage for 1 year
Heat stability	30°C:  70% of the lytic activity is lost after storage for 3 months 90% of the lytic activity is lost after storage for 3 months 60°C:   Lytic activity is lost on incubation for 5 minutes
Specificity (Lytic Spectrum)	Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc.

(※1) See reference, Kitamura, K., Kaneko, T., Yamamoto, Y., J. Gen. Appl. Microbiol., 18, 57 (1972) as to the definition of each enzyme units.

Unit Definition

One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A800) of the reaction mixture under the following condition.

Reaction Mixture

Enzyme solution	1 ml (0.05–0.1 mg/ml for Zymolyase)
Brewer's yeast cell suspension	3 ml (2 mg/ml)
1/15M Phosphate buffer	5 ml (pH7.5)
Distilled water	1 ml

After incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer’s yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer’s yeast. 

Preparation of 1/15 M Phosphate Buffer (1L of pH 7.5 buffer)

1. Dissolve 19.1 g of di-sodium hydrogenphosphate 12-hydrate in distilled water to make an 800 mL solution.
2. Dissolve 1.814 g of potassium dihydrogenphosphate in distilled water to make a 200 mL solution.
3. Mix the two solutions.

The below phosphate buffers (pH 7.5) have been tested for compatibility.   

Specifications

Product Name

Zymolyase, Arthrobacter luteus, CAS No.37340-57-1

Synonyms

Zymolyase, Arthrobacter luteus | Yeast lytic enzyme | Lyticase

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Arthrobacter luteus; ≥20000 U/g enzyme powder

Bioactivity

≥20000 U/g enzyme powder

Action Type

INHIBITOR

CAS

37340-57-1

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Arthrobacter luteus; ≥20000 U/g enzyme powder

Reconstitution

Reconstitute in 1/15 M Phosphate buffer, pH7.5.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term. Upon reconstitution, it is recommended to aliquot.

Unit definition

One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm (A800) of the reaction mixture under the following condition.