3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA - peroxidase substrate

Cat. No.: T117926

GRADE & PURITY peroxidase substrate

Synonyms

TMB

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

ELISA

Overview

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Specifications

Synonyms

TMB

Specifications & Purity

peroxidase substrate

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: light Sensitive

References

1. Luyao Zhao, Jin Liu, Bing Bai, Yongpeng An, Yukun Yang, Xiaowen Wang, Xu Jing.  (2024)  Natural deep eutectic solvent-based QuEChERS followed by magnetic beads ELISA for the analysis of ochratoxin A in cereal samples.  FOOD CONTROL,      [PMID:] [10.1016/j.foodcont.2024.110384]