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Aladdin™ Immobilized Reductant Column, 2 mL
AVAILABLE TO ORDER
Storage
Store at 2-8°C
Shipped In
Wet ice
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Why this grade
for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product introduction：
Immobilized Reductant Column contains beaded resin to which an thiol-based reducing agent has been immobilized to enable solid-phase reduction of peptide and protein disulfide bonds.
Features of the Immobilized Reductant Column:
• Eliminates soluble components which interfere with the assay of free thiol groups
• No need to remove reducing agent after the reaction
• Reduced protein, free of contaminating thiols, can be easily assayed using the BCA method
• Solid phase; protocols for both peptides and proteins
• Excellent protein recovery
• Regenerable matrix useable up to four times
Both the mercaptan and the DTT methods of reduction use soluble reducing agents. This requires that the reducing reagents be removed from the sample before further procedures can be performed. Measurements of free sulfhydryl content cannot be made in the presence of these agents, and they must be removed prior to crosslinking. Removal of a soluble reductant can be difficult when a peptide is involved, and extensive desalting may result in protein loss or extreme dilution when small quantities of protein are reduced.
Pierce Immobilized Reductant Columns were developed to eliminate these difficulties, and are highly effective for reducing disulfide bonds.Immobilized TCEP Disulfide Reducing Gel is also available for reduction of peptides and proteins without contaminating your sample with reducing agent.
• Form:Liquid
Features of the Immobilized Reductant Column:
• Eliminates soluble components which interfere with the assay of free thiol groups
• No need to remove reducing agent after the reaction
• Reduced protein, free of contaminating thiols, can be easily assayed using the BCA method
• Solid phase; protocols for both peptides and proteins
• Excellent protein recovery
• Regenerable matrix useable up to four times
Both the mercaptan and the DTT methods of reduction use soluble reducing agents. This requires that the reducing reagents be removed from the sample before further procedures can be performed. Measurements of free sulfhydryl content cannot be made in the presence of these agents, and they must be removed prior to crosslinking. Removal of a soluble reductant can be difficult when a peptide is involved, and extensive desalting may result in protein loss or extreme dilution when small quantities of protein are reduced.
Pierce Immobilized Reductant Columns were developed to eliminate these difficulties, and are highly effective for reducing disulfide bonds.Immobilized TCEP Disulfide Reducing Gel is also available for reduction of peptides and proteins without contaminating your sample with reducing agent.
• Form:Liquid
Specifications
Storage
Store at 2-8°C
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →✅ Certificate of Analysis (COA)
Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →🔬 Specification Sheet
Full quality attributes and acceptance criteria for this grade.
View spec sheet →Advanced Data
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews
Customer Reviews
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