Beta-1,4-galactosyltransferase 1

Cat. No.: B755120

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, His Tag, expressed in Baculovirus-BTI-TN-5B1-4 Cells；>2000 U/mg protein；Protein concentration: See COA

Accession #

P08037

Protein Tag

C-His

Expression system

Baculovirus-BTI-TN-5B1-4 Cells

Bioactivity

>2000 U/mg protein

Overview

 Bovine Beta-1,4-galactosyltransferase 1 (Bovine B4GALT1) is a type II transmembrane glycoprotein belonging to the glycosyltransferase family 7. It is commonly involved in the synthesis of glycoconjugates. Using β-1,4-galactosyltransferase (B4GALT1) and UDP-galactose (UDP-Gal) as the donor, in vitro galactosylation can be performed on all GlcNAc1-2Man units of complex molecules such as glycoproteins and monoclonal antibodies (mAbs).

Product Properties

Source: Baculovirus-BTI-TN-5B1-4 Cells

Label: C-terminal His Tagged

Molecular weight: 49.5 kDa

Storage Buffer: 20 mM Tris-HCl, 200 mM NaCl, 20% Glycerin, pH 7.4

Enzyme activity: >2000 U/mg protein

Unit Definition: The amount of enzyme (mg) required to transfer 1 nmol of galactose to GlcNAc per minute at 37℃.

Transport and Storage Methods

Transported on ice packs or dry ice; stored at -20℃; valid for 12 months.

Recommended Usage (can be adjusted according to actual experimental conditions)

1. Reaction System: 10 mM MnCl₂, 50 mM Tris-HCl, pH 6.5

2. Reaction Reagents: UDP-Gal:GlcNAc (equivalent ratio) ≥1:1

3. Prepare the reaction solution as described above, add an appropriate amount of enzyme. After the reaction at 37°C is completed, inactivate the enzyme by incubating in a hot water bath (≥85°C) for 10 minutes.

Precautions

Please pay attention to experimental safety and operate with gloves. This product is for research use only!

Specifications

Product Name

Beta-1,4-galactosyltransferase 1

Synonyms

B4GALT1 | GT1 | GTB | CDG2D | GGTB2 | CLDLFIB | B4GAL-T1 | beta4Gal-T1

Grade

ActiBioPure™, Bioactive, High Performance, His Tag, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, His-Tag, expressed in Baculovirus-BTI-TN-5B1-4 Cells；>2000 U/mg protein；Protein concentration: See COA

Biochemical and Physiological Mechanisms

Beta-1, 4-galactosyltransferase 1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carb

Bioactivity

>2000 U/mg protein

Accession #

P08037

Predicted molecular weight

49.5 kDa

Enzyme Commission Number

EC 2.4.1.38

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in Baculovirus-BTI-TN-5B1-4 Cells；>2000 U/mg protein；Protein concentration: See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme (mg) required to transfer 1 nmol of galactose to GlcNAc per minute at 37℃.