Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500×) - sterile-filtered,BioReagent,500×

Cat. No.: C1508365

GRADE & PURITY Sterile-filtered, BioReagent, 500×

Synonyms

Cell Stimulation Cocktail (500X) | Cell Activation Cocktail (without Brefeldin A)

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Flow Cytometry

Overview

Product Introduction

This product is a classic 500X concentrated cell stimulation cocktail containing an optimized ratio of Phorbol 12-myristate 13-acetate (PMA) and Ionomycin. The synergistic action of these components provides robust activation of immune cells such as T cells and NK cells, inducing gene expression and natural secretion of multiple cytokines (e.g., IFN-γ, TNF-α, IL-2). This cocktail does NOT contain protein transport inhibitors like Brefeldin A or Monensin, making it ideal for studying natural cytokine secretion kinetics, signal transduction pathways, and assays requiring supernatant collection.

Key Components:

• Ethonal 
•  PMA 25μg/mL， lonomycin 500μg/mL

Applications

• Immune cell activation for ELISA, ELISpot, or multiplex immunoassays (requires supernatant analysis).

• Functional assessment of immune cells (e.g., T cell activation, proliferation).

• Cell signaling studies (PKC and calcium signaling pathways).

• Important: For intracellular cytokine staining and flow cytometry analysis, protein transport inhibitors MUST be added separately during stimulation (please use the version with protein transport inhibitors, Cat# C1508366).

Instructions for Use

Standard Stimulation Protocol

• Dilution: Dilute the cocktail 1:500 in complete culture medium immediately before use. For example, add 2 µL of the cocktail to 1 mL of cell suspension.
• Stimulation: Incubate cells in a 37°C, 5% CO₂ incubator.
• For secretion/gene expression assays: A 4-6 hour stimulation is recommended.

• For long-term stimulation/supernatant collection: Can be extended to 16-48 hours (please optimize the duration).

Key Precautions

• Optimization of stimulation time (4-48 hours) is recommended for first-time use or new cell systems.
• Prolonged stimulation (>24 hours) may affect cell viability and phenotype. Pre-experiment evaluation is advised.
• This product lacks protein transport inhibitors. For ICS, inhibitors must be added for the final 4-6 hours of stimulation.
• Aliquot upon receipt to minimize freeze-thaw cycles.

Specifications

Synonyms

Cell Stimulation Cocktail (500X) | Cell Activation Cocktail (without Brefeldin A)

Specifications & Purity

sterile-filtered, BioReagent, 500×

Stability And Storage

Aliquot upon receipt and store frozen at -20°C(12 months). Protect from light. Avoid repeated freeze-thaw cycles.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered