Concanavalin A from Canavalia ensiformis (Jack bean), CAS No.11028-71-0

CAS: 11028-71-0 Cat. No.: C755208 EC Number: 234-258-2

GRADE & PURITY Type IV, lyophilized powder

Overview

Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.
Quality
Highly purified by specific affinity chromatography
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Purpose
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
for the stimulation of spleen in mitogen assays

Specifications

Product Name

Concanavalin A from Canavalia ensiformis (Jack bean), CAS No.11028-71-0

Specifications & Purity

Type IV, lyophilized powder

Biochemical and Physiological Mechanisms

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetrame

CAS

11028-71-0

Molecule Type

Protein

Storage and Shipping

Concentration

Type IV, lyophilized powder

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Nucleosides, nucleotides, and analogues
    • Class Pyrimidine nucleosides
      • Subclass Pyrimidine 2',3'-dideoxyribonucleosides

• Kingdom: Organic compounds
• Superclass: Nucleosides, nucleotides, and analogues
• Class: Pyrimidine nucleosides
• Subclass: Pyrimidine 2',3'-dideoxyribonucleosides
• Intermediate Tree Nodes: Not available
• Direct Parent: Pyrimidine 2',3'-dideoxyribonucleosides
• Alternative Parents: Benzenesulfonamides Benzoic acids Benzenesulfonyl compounds Benzoyl derivatives Pyrimidones Organosulfonamides Hydropyrimidines Vinylogous amides Tetrahydrofurans Heteroaromatic compounds Aminosulfonyl compounds Ureas Lactams Azo imides Azo compounds Oxacyclic compounds Monocarboxylic acids and derivatives Carboxylic acids Azacyclic compounds Primary alcohols Organopnictogen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Not available
• Substituents: Pyrimidine 2',3'-dideoxyribonucleoside - Benzenesulfonamide - Benzenesulfonyl group - Benzoic acid - Benzoic acid or derivatives - Benzoyl - Pyrimidone - Benzenoid - Organosulfonic acid amide - Pyrimidine - Hydropyrimidine - Monocyclic benzene moiety - Heteroaromatic compound - Vinylogous amide - Aminosulfonyl compound - Tetrahydrofuran - Sulfonyl - Organosulfonic acid or derivatives - Organic sulfonic acid or derivatives - Urea - Lactam - Azo imide - Azo compound - Oxacycle - Azacycle - Organoheterocyclic compound - Monocarboxylic acid or derivatives - Carboxylic acid - Carboxylic acid derivative - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Primary alcohol - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Alcohol - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as pyrimidine 2',3'-dideoxyribonucleosides. These are compounds consisting of a pyrimidine linked to a ribose which lacks a hydroxyl group at positions 2 and 3.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

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