How should the experimental groupings/controls be set up?

How should the experimental groupings/controls be set up?

Blank control: used to set the voltage of each channel.
Isoform control: Isoform control antibody is essential for the determination of negative and positive cells, especially for the indicators of low or continuous expression, which are not good for data analysis.
Single-tube control: In flow polychromatography, if there is interference between the fluorescence of each channel, it is necessary to make a separate single tube for each fluorescence to adjust the compensation.
FMO control: FMO control, also known as fluorescence minus one control, refers to the multicolor experiment, to detect the expression of a fluorescence channel, due to the fluorescence leakage of the influence of the main, so the detection of the channel's antibody is first removed, to detect the leakage of the other channels to the channel's background fluorescence level, to correctly exclude, thus helping to set up the door.

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