Solubilization and preservation of RNA in formamide
Storing RNA in formamide has many advantages over storing it in water or ethanol: (1) formamide will protect the RNA from degradation by RNA enzymes, and RNA st
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Oligodeoxythymidine cellulose purification band poly( A)+RNA
Most mammalian messenger RNAs (mRNAs) carry a poly(A)+ tail, and Poly(A)+-carrying RNAs can be isolated from total RNA using this protocol.
In vivo isolation of ribonucleoprotein complex by immunoprecipitation-rPCR of RNA
The RNA Immunoprecipitation-Randomized Polymerization Chain Reaction method can be used to identify RNA components in intracellular RNP complexes. This experime
Experimental purification and removal of nucleoprotein complexes by antisense affinity chromatography
This experiment was derived from "RNA Laboratory Guidebook", edited by Xiaofei Zheng.
Experiments on the preparation of inhomogeneous ribonucleoprotein complexes
In the nucleus, the transcription products of RNA polymerase II bind to a large number of different types of nuclear precursor mRNA/mRNA-binding proteins, heter
Biotinylated oligodeoxythymidine-streptavidin magnetic bead purification band Poly(A)+RNA
The use of magnetic microspheres instead of transferring fiber cords as a medium is more commonly used and has been commercialized.
deoxyribonuclease assay
With the development of in vitro molecular experimental techniques, many DNA molecules with different catalytic functions have been discovered in recent years.
Experimental detection of protein-double-stranded RNA interactions by methylene blue-mediated photocrosslinking reaction
Methylene blue-mediated photocrosslinking reactions are a complement to UV crosslinking reactions. Methylene blue belongs to the phenothiazine group of dyes. Du
Fungal RNA extraction
Fungal RNA extraction can be: (1) used in the study of fungal RNA interference mechanism; (2) used in cDNA library construction and other studies; (3) used in t