Northern blotting and narrow line hybridization analysis of RNA
Northern blot is one of the most rigorous methods to study gene expression, which can quantitatively analyze the expression abundance of a specific mRNA in tiss
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cDNA synthesis technology
After the mRNA of the target gene has been identified by in vitro cell-free translation, the first strand of cDNA can be synthesized in the steps described in t
Isolation of cytoplasmic RNA from mammalian cells
The following method is a simple and effective method for purifying cytoplasmic RNA from tissue culture cells, which appeared at an earlier stage of RNA isolati
Viral RNA Extraction
Viral RNA extraction can be (1) used for the study of RNA viral replication mechanisms, (2) used for reverse genetics studies, and (3) used for other studies in
Total RNA extraction from egg and embryo cells
This method is a simple and effective way to isolate RNA from oocytes, fertilized eggs and fetal cells of frogs, sea urchins, periphyton, worms and flies.
Whole blood RNA extraction
Whole blood RNA extraction can be used (1) to study the mechanism of RNA interference, etc.; (2) as a template for reverse transcription; and (3) for other stud
Construction of siRNA expression vectors
The construction of siRNA expression vectors can be applied to: (1) as a powerful tool for gene function analysis in the post-genomic era; (2) genomics and cell
Extraction of RNA from cultured cells by the CsCl method
RNA preparation by guanidine thiocyanate extraction and cesium chloride centrifugation is suitable for isolation of RNA from cells with difficult separation of
Rapid isolation of total RNA from mammalian cells
SDS lyses the cells and extracts the total RNA by acid phenol graded extraction, which is a suitable method for treating cells with relatively low mRNA content