Plant RNA preparation experiment
Since there are many sources of RNA species, there are different methods of extraction and preparation, generally phenol method, detergent method and guanidine
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Yeast Spliceosome Analysis Experiment
A precursor is a macromolecular ribonucleoprotein complex that splices mRNA precursors in the nucleus of eukaryotic cells. It consists of five snRNPs and a larg
In vitro splicing analysis experiments in mammalian cells
It is typical for splicing reactions to use nuclear extracts, i.e., S100 extracts complementing partially purified fractions of SR proteins or crude extracts, m
RNA extraction and labeling experiments
None
Chromosome preparation experiments on cultured peripheral blood cells
Although chromosomes can be prepared from other cells, human peripheral blood leukocytes are the easiest to synchronize, allowing high-resolution analysis of th
Cytoplasmic RNA extraction
Since there are many sources of RNA species, there are different methods of extraction and preparation, generally phenol method, detergent method and guanidine
Fungal RNA extraction
Fungal RNA extraction can be: (1) used in the study of fungal RNA interference mechanism; (2) used in cDNA library construction and other studies; (3) used in t
Experiments on the isolation and analysis of pre-splitters and spliceosomes
The spliceosome is a ribonucleoprotein body (RNP) composed of RNA and proteins, which removes introns from precursor mRNA during the splicing process of precurs
Principles, materials and procedures for denaturing agarose gel electrophoresis of RNA
This experiment is mainly used to determine the integrity of RNA extracts.