RNA radiolabeling
Intramolecular labeling of RNA during transcription, post-transcriptional labeling of the 5' end of RNA using T4 polynucleotide kinase and labeling of the 3' en
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Ligation of RNA molecules with T4 RNA ligase
T4RNA ligases have been used to generate many site-specific modified RNAs, especially oligonucleotide-modified and tRNA-modified with antisense codon tRNAs.In a
T4 DNA ligase ligates RNA molecules
T4 DNA Ligase joins double-stranded complexes, including RNA-DNA heteroduplexes and RNA-RNA heteroduplexes, defectively.
Deoxyribonuclease cleaves RNA at a specific site
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
A simple and effective transcription method to reduce heterogeneity at the 3' end of RNA
Ribosyl C2'-methoxy modification of the nucleotides at the 2 5' ends. Amplification of DNA by PCR with modified primers still reduces N+l activity, and since PC
mRNA 3' end processing in vitro
There are two strategies for in vitro experiments; one is to co-incubate a plasmid containing the target gene at the Po ly( A) locus with cell-free extracts to
Translation of mRNA in wheat germ extracts
Wheat germ extract in vitro translation system for efficient translation of mRNA in viruses and eukaryotic cells in a heterogeneous cell-free system
Translation of mRNA in reticulocyte lysates
The mRNA extracted from mammalian cells or produced by in vitro transcription of cloned DNA can be translated in cell-free extracts to synthesize proteins, whic
An in vitro translation system for African Xenopus oocytes
The African Xenopus oocyte in vitro translation system is capable of sustained modification and processing of most exogenous mRNAs due to its ability to translo