RNA extraction from fresh and frozen blood
Whole blood contains nucleated leukocytes, so it is easy to extract RNA from blood, but red blood cells are rich in ribonuclease, so it is difficult to isolate
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Extraction and isolation of rat liver rRNAs
Rat liver was homogenized, the supernatant was centrifuged, and RNA was extracted with a mixture of phenol and m-cresol. rRNA and mRNA were separated in the pre
Extraction of RNA from tissues by the CsCl method
Because some tissues (e.g., pancreas and uterus) contain high concentrations of endogenous RNAases, more care must be taken in purifying tissue-derived RNA.
Synthesize 5' capped transcripts
Some RNA transcripts require an m7 G(5')Ppp(5')G cap at the 5' end, and they have higher translational efficiency only in cell-free extracts or in African Xenop
Rapid isolation of total RNA from mammalian cells
SDS lyses the cells and extracts the total RNA by acid phenol graded extraction, which is a suitable method for treating cells with relatively low mRNA content
Extraction of RNA from cultured cells by the CsCl method
RNA preparation by guanidine thiocyanate extraction and cesium chloride centrifugation is suitable for isolation of RNA from cells with difficult separation of
Deoxyribonuclease cleaves RNA at a specific site
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
RNA reverse transcription
The RNA template is used to obtain DNA by reverse transcription, which is then used to replicate and infect cells.
RNA radiolabeling
Intramolecular labeling of RNA during transcription, post-transcriptional labeling of the 5' end of RNA using T4 polynucleotide kinase and labeling of the 3' en