Cell fixation sectioning and embedding experiments
Within this section we will present some basic techniques that are applicable to most histological studies, which can be appropriately categorized as fixation,
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somatic cell hybridization assay
With the advent of molecular genetics techniques, important advances have been made in somatic cell hybridization: the DNA used as a probe can be obtained from
Mesenchymal Stem Cells (MSCs) Identification
MSCs in culture are best assessed by clone formation assays and trilineage differentiation capacity toward osteoblasts, adipocytes, and chondrocytes. However, c
Single nerve cell labeling assay
In 1949 Ling and Gerard used capillary glass tubes to make microelectrodes so that one could record the electrical activity of individual nerve cells before lab
Purification and culture of oligodendrocytes by oscillating shaker method
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Basic principles of culture and characterization of bone marrow stromal-derived mesenchymal stem/progenitor cells (MSCs)
Source: Human Stem Cell Culture
Single nucleated cells (MNCs) acquisition assay
Source: Human Stem Cell Culture
Ultrastructural experiments on cells
Although electron microscopy cannot observe living cells and tissues, the advent of electron microscopy still revolutionized the development of microscopy. Sinc
Experiments on the preparation of encapsulated tissue culture plates
This experiment describes the preparation of cutin A-encapsulated culture plates for growing CEF monolayers for in vivo immunostaining. Source Compact Molecular