Experiments for the determination of growth fraction
Cells were labeled continuously for 48h and sampled at intervals for radioautography. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
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Radiation autoradiography in in situ hybridization
Isotope-labeled probes were hybridized with cells fixed on microscope slides, and then the hybridization sites were visualized by radiographic autoradiography.
Expansion of insect cells
Cells were mechanically isolated from the cell monolayer and cultured for expansion at 27°C and in suspension.
Electronic cell counting by means of a resistor
Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc
Real-Time Label-Free Dynamic Cell Analysis
RTCA real-time detection of live cell activity
cell hybridization
When fusing cells in suspension or monolayer cell systems, the treatment of cells with PEG should be short to minimize cell death. Usually, a 24 h recovery peri
time-lapse camera
Cells are cultured in culture chambers with suitable optical microscope characteristics. The microscope is fitted with a camera connected to a gas recorder, an
Fluorescence in situ hybridization using single-copy genomic probes and chromosome coloring methods
Hybridization of biotin-labeled or digoxigenin-labeled probes to chromosomes detects degenerate chromosomes, and individual probes can be detected by double-ant
Stimulation of lymphocytes with PHA
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)