Cold Trypsin Dissociation of Tissues
The cut tissues were placed in trypsin at 4°C for 16-18 h. After removing the trypsin, the cells were warmed and dispersed in warm medium.
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Liposome-mediated transient transfection
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Collagenase dissociation of tissues
Chopped tissues were placed in complete medium containing collagenase for incubation, and after the tissues were broken down, collagenase was removed by centrif
Isolation and culture of single muscle fibers from skeletal muscle
Grind whole skeletal muscle treated with collagen to disperse into single muscle fibers. Cultivate single muscle fibers on Matrigel and collect the migrating ce
Culture of chondrocytes with alginate microbeads
Alginate microbead cultures based on the gelling action of calcium chloride in chondrocyte alginate suspensions.
Monolayer culture cells changing culture medium experiment
Observe the culture with the naked eye and inverted microscope, and if indicated, e.g., decreased pH, remove the old medium and add as fresh medium, then place
chicken embryo organ primordium
Single organs or tissues were cut out and placed whole in cold trypsin overnight, trypsin was removed, organs or tissues were briefly incubated, cells were disp
Preparation of the feeding layer
Inoculation of homologous or heterologous cells-such as those from mouse embryos. Pending clonal culture of the cell under test, medium density, irradiated with
Enrichment of living cells
6 ml of Ficoll-Hypaque solution was added to a 25 ml bind-cap centrifuge tube, 9 ml of medium containing 2 × 107 cells was added on top, centrifuged, and