Transmission of monolayer cells
The medium was removed, trypsin was added for brief action, incubated, cells were dispersed in medium, counted, diluted and re-inoculated.
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Selective culture of primary tumor cells: confluent feeder layer tumor cell screening assay
The feeder layer technique depends on the prevention of fibroblast overgrowth through other contacts that inhibit cell formation in the monolayer. The feeder la
Preparation of conditioned media
Collect medium from homologous or heterologous cell lines in late logarithmic growth, filter, and dilute with fresh medium as needed.
Clonal culture by dilution
Cells are inoculated at low density, cultured until colony formation, stained (for clone formation rate and survival assays ) or used for screening when cells a
Pancreatic epithelial cell isolation and culture experiments
This protocol is really to culture the isolated and obtained pancreatic vesicle epithelial cells in collagen-coated petri dishes. In turn, two-dimensional aggre
Rat hepatocyte isolation assay
Although cells isolated from adult livers do not express all the properties of the liver parenchyma, there is no doubt that suitable cell lines can be cultured.
Mechanical separation using screens
The tissue in the medium is forcefully passed through a series of sieves of decreasing pore size until a desirable suspension of single cells or small tissue ma
Suspension cell passaging
Samples were aspirated from suspension cell cultures, cells were counted, and appropriate amounts of suspension cells were inoculated into fresh medium in new c
Corneal epithelial cell culture experiment
There has been a great deal of interest in forming corneas in culture, as attempts have been made to replace the rabbit eye experiments (Draze's experiments) wi