Freund's Incomplete Adjuvant , CAS No.F393371

CAS: F393371 Cat. No.: F393371

Synonyms

FICA

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Overview

Only used for antibody preparation and animal immunology related research, not for human or drug use.

Application：

1. Freund's adjuvant can be used as an oil in water emulsion for producing immunogens. The antigens in oil-in-water emulsions can stimulate high and persistent antibody responses, which can be attributed to the slow release of antigens.
2. Freund's (incomplete) adjuvant lacks the mycobacteria found in Freund's complete adjuvant, so it can minimize side effects. It is used to enhance injection. It is used to emulsify antigens injected into animal bodies, thereby producing monoclonal antibodies in the antiserum.

Explanation：

1. Immunity: This product can quickly form a water in oil state when mixed with antigen. Stable lotion can be formed under the conditions of 1:1-1:2. 1:2 emulsification is thicker than 1:1 emulsification. After emulsification, water will not disperse for several weeks; The immune effect is excellent after testing multiple types of antigens and animal genera, due to the milk
Thoroughly and firmly solidified, it is less likely to exhibit immune tolerance compared to other adjuvants.
2. Preparation of monoclonal antibody ascites: Adjuvants are injected into the abdominal cavity of BALB/C mice, with each mouse treated with 0.3-0.5ml for about 15 days (usually 12-18 days), and then hybridoma cells are injected. It is recommended to inject 800000-100000 cells per mouse. Ascites may be delayed by 1.5-2 days compared to regular adjuvants, but there is no significant difference
Under normal circumstances, the amount of ascites is 1.5-2 times that of a regular adjuvant, and the efficacy of monoclonal antibodies in ascites remains unchanged.  

Specifications

Synonyms

FICA

Storage

Store at 2-8°C, Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: Light sensitive

References

1. Jia-Hui Ma, Li-Qiu Ren, Tian-Xuan Tang, Yu-Yao Chen, Chen-Xi Zhang, Yu-Feng Ke, Yan Zhang, Mitchell N. Muskat, Xiang-Rong Cheng.  (2024)  Effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104381]

2. Xiaofang Li, Jinyan Li, Yongwei Feng, Liqiang Liu, Hua Kuang, Chuanlai Xu, Lingling Guo.  (2024)  Fluorescent microsphere immunochromatographic sensor for the detection of total fumonisins B1, B2, and B3 in grain samples.  JOURNAL OF FOOD COMPOSITION AND ANALYSIS,      [PMID:] [10.1016/j.jfca.2024.106018]

3. Qianqian Lu, Liqiang Liu, Jinyan Li, Shanshan Song, Hua Kuang, Chuanlai Xu, Lingling Guo.  (2024)  Rapid and sensitive quantitation of amitraz in orange, tomato, and eggplant samples using immunochromatographic assay.  FOOD CHEMISTRY,      [PMID:38452506] [10.1016/j.foodchem.2024.138899]

4. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104936]