Gly-Phe-AMC, TFA salt - BioReagent, ≥98% , CAS No.201852-70-2(free base)

CAS: 201852-70-2(free base) Cat. No.: G1456329 Molecular Weight: 379.4(free base)

GRADE & PURITY BioReagent, ≥98%

Synonyms

GF-AMC | H-Gly-Phe-AMC | L-Phenylalaninamide, glycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- | (2S)-2-[(2-aminoacetyl)amino]-N-(4-methyl-2-oxochromen-7-yl)-3-phenylpropanamide | (2S)-2-[(2-aminoacetyl)amino]-N-(4-methyl-2-oxo-chromen-7-yl)-3-phenyl-propa

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Enzyme activity assay

Overview

Gly-Phe-AMC is a synthetic dipeptide composed of glycine (Gly) and phenylalanine (Phe), and is linked to the fluorescent group AMC (7-amino-4-methylcoumarin). This structure gives it specific chemical properties and biological activities.
Uses:
Enzyme activity detection: Gly-Phe-AMC is a sensitive substrate for dipeptidyl aminopeptidase I (cathepsin C). Under the action of the enzyme, it can be cleaved to release the AMC fluorescent group, thereby realizing real-time monitoring of enzyme activity. This characteristic enables it to have wide applications in biochemical research, especially in enzyme activity detection and enzyme inhibitor screening.
Biological research: In addition, Gly-Phe-AMC can also be used to study biological processes such as protein-protein interactions, protein modifications, and signal transduction pathways. By cleverly designing experiments, researchers can use this reagent to observe and analyze the dynamic changes of specific proteins in cells and their interactions with other molecules.

Specifications

Synonyms

GF-AMC | H-Gly-Phe-AMC | L-Phenylalaninamide, glycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- | (2S)-2-[(2-aminoacetyl)amino]-N-(4-methyl-2-oxochromen-7-yl)-3-phenylpropanamide | (2S)-2-[(2-aminoacetyl)amino]-N-(4-methyl-2-oxo-chromen-7-yl)-3-phenyl-propa

Specifications & Purity

BioReagent, ≥98%

Stability And Storage

Store at -20℃ long term (24 months).  Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Storage

Protected from light, Store at -20°C, Desiccated, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent

Purity

≥98%

Names and Identifiers

• Molecular Weight: 379.4(free base)

Chemical and Physical Properties

• Solubility: Soluble in H2O.