HMGB1 Mouse mAb - Anticorps primaire, specific to HMGB1, IgG2b

Cat. No.: Ab156561

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, See COA

Application

Flow Cytometry, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG2b

Clone Number

AB02/4A1

Conjugation

Unconjugated

Vue d’ensemble

Application
WB: 1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa
Flow: 0.02 μg/mL - 2.5 μg/mL

Specifications

Product Name

HMGB1 Mouse mAb - Anticorps primaire, specific to HMGB1, IgG2b

Synonymes

Amphoterin antibody | Chromosomal protein, nonhistone, HMG1 antibody | DKFZp686A04236 antibody | Heparin-binding protein p30 antibody | High mobility group 1 antibody | High mobility group box 1 antibody | High mobility group protein 1 antibody | High mob

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

Espèce hôte

Souris

Spécificité

HMGB1

Immunogène

Recombinant full length protein corresponding to Human HMGB1

Contrôle positif

WB: HEK293, HeLa, HepG2, Jurkat, MCF-7, A549, A431, NIH/3T3 and C6 whole cell lysates Flow：HeLa cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

IgG2b

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: HMGB1 Mouse mAb (Ab156561)

HMGB1 Mouse mAb (Ab156561) - Western Blot
All lanes: HMGB1 Antibody (Ab156561) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 24.9 kDa
Observed band size: 27.2 kDa
Exposure time: 118.1 s 

Western Blot: HMGB1 Mouse mAb (Ab156561)

HMGB1 Mouse mAb (Ab156561) - Western Blot
All lanes: HMGB1 Antibody (Ab156561) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 24.9 kDa
Observed band size: 26.1 kDa
Exposure time: 27.8 s 

ICC/IF: HMGB1 Mouse mAb (Ab156561)

HMGB1 Mouse mAb (Ab156561) - ICC/IF
IF analysis of HMGB1 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HMGB1 Mouse mAb (Ab156561) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application

Dilution info

WB

1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa

Flow

0.02 μg/mL – 2.5 μg/mL

WB

1/2,000 - 1/50,000; Predicted molecular weight: 25.0 kDa

WB

1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa

Flow Cytometry

0.02 μg/mL - 2.5 μg/mL

Cibles associées (humaines)

HMGB1 Tchem High mobility group protein B1 (0 Activities)

Discover Target: HMGB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TCF7 Tbio Transcription factor 7 (0 Activities)

Discover Target: TCF7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HMGB1 Tchem High mobility group protein B1 (86 Activities)

Discover Target: HMGB1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HMGB1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.