Pepsin−Agarose from porcine gastric mucosa

Cat. No.: P755312

GRADE & PURITY lyophilized powder,≥30 units/mg dry solid

Accession #

P00791

Overview

Pepsin is an enzyme found in gastric secretion.

Application:

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.

Used to produce F(ab′)₂ fragments of antibodies.¹

Specifications

Product Name

Pepsin−Agarose from porcine gastric mucosa

Specifications & Purity

lyophilized powder, ≥30 units/mg dry solid

Biochemical and Physiological Mechanisms

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Accession #

P00791

Enzyme Commission Number

3.4.23.1(BRENDA, IUBMB)

Molecule Type

Enzyme

Storage and Shipping

Concentration

lyophilized powder,≥30 units/mg dry solid

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads