GRADE & PURITYSterile-filtered?Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.5mg/mL
sterile-filtered,BioReagent,5mg/mL BioReagent,Sterile-filtered for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
This product is a ready-to-use, sterile-filtered concentrate of Phytohemagglutinin-P (PHA-P). PHA-P is derived from red kidney beans (Phaseolus vulgaris) and represents the protein form of PHA prior to the separation of subunits, containing a mixture of glycoproteins with mitogenic and agglutinating activities. Provided as a 500X concentrated solution, it is designed for convenient direct dilution in cell culture to efficiently induce mitosis and proliferation of human and mouse peripheral blood mononuclear cells (PBMCs).
Usage Protocol
500X concentrate. The recommended final stimulation concentration ranges from 1-10 μg/mL, depending on cell type and experimental system; optimization via pilot experiments is advised.
Primary Applications
Nonspecific activation and proliferation assays of T lymphocytes.
Culture of peripheral blood mononuclear cells (PBMCs) and induction of cytokine secretion.
Lymphocyte transformation tests.
Positive control stimulant in immune cell functional studies (e.g., ELISPOT, flow cytometric cytokine detection).
Specifications
Synonyms
PHA-P
Specifications & Purity
sterile-filtered, BioReagent, 5mg/mL
Stability And Storage
Aliquot upon receipt and store frozen at -20°C(12 months). Avoid repeated freeze-thaw cycles.
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Human peripheral blood lymphocytes were stimulated with Phytohemagglutinin-P (PHA-P) Solution (500X) (P1508369) for 3 days. The cells were then stained with Recombinant IL-2 Receptor alpha/CD25 Antibody (APC) (Ab230835) (red) compared to an isotype control (blue). Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody. Unstimulated PBMCs were used as control (left panel).
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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