Recombinant Alkali-tolerant Protein A, CAS No.91932-65-9

CAS: 91932-65-9 Cat. No.: rp192268 EC Number: 295-242-9 PubChem CID: 363902062

GRADE & PURITY Animal Free, Carrier Free, PBS Only, ≥90%(HPLC), Dimer≤2.0%(SEC-HPLC)

Expression System

E.coli

Accession #

P02976

Protein Tag

No tag

Expression system

E. coli

Endotoxin Concentration

<5.0 EU/mg

Overview

This product is a freeze-dried powder of recombinant alkali resistant protein A expressed by Escherichia coli and purified by chromatography and freeze-drying. Aladdin recombinant protein A is a modification of the protein structure based on natural Staphylococcus aureus protein A. It consists of four Fc binding domain fragments linked together, with a molecular weight of 27KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant protein A can be bonded to agarose gel to prepare an affinity filler for antibody purification. It can separate and purify antibodies of different subtypes of mammals or genetic engineering recombinant proteins containing Fc fragments of antibodies from ascites, serum, cell culture supernatant or cell extracts. The C-terminal of Aladdin recombinant alkali resistant protein A contains a cysteine, which can enhance its directional covalent bonding with gel.
2、Purpose
Biochemical research: used for immune analysis and purification of IgG. 
Preparation of affinity fillers for antibody purification: Multiple mammalian subtypes of antibodies or genetically engineered recombinant proteins containing antibody Fc fragments can be isolated and purified from ascites, serum, cell culture supernatant, or cell extracts.
3、Recommended usage
This product is a recombinant expression of alkali resistant protein A in Escherichia coli. Its C-terminus is designed with a cysteine residue, which can be coupled through the thiol non epoxy group on it to prepare affinity chromatography fillers. The activation of epoxy groups and protein A coupling methods need to be determined through experiments.

Specifications

Product Name

Recombinant Alkali-tolerant Protein A, CAS No.91932-65-9

Synonyms

Protein A | Staphylococcal Protein A | Staphylococcal Protein A Recombinant | Immunoglobulin G-binding protein A | gG-binding protein A | Immunoglobulin G binding protein A | SPA | rat-PA

Grade

Animal Free, Carrier Free, PBS Only

Specifications & Purity

Animal Free, Carrier Free, PBS Only, ≥90%(HPLC), Dimer≤2.0%(SEC-HPLC)

Biochemical and Physiological Mechanisms

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immu

Endotoxin Concentration

<5.0 EU/mg

Expression System

E.coli

Accession #

P02976

Predicted molecular weight

27 kDa

SDS-PAGE

24.3-29.7 kDa, under reducing conditions

CAS

91932-65-9

Molecule Type

Protein

Storage and Shipping

Concentration

Dimer≤2.0%(SEC-HPLC)

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

References

1. Li Yangyang, Zhu Zhengwei, Qu Wenli, Yang Qing, Liu Yan, Wang Qiao, Duan Shuo, Wu Jine, Gong Zhiyong, Xu Lin.  (2023)  A highly sensitive immunochromatographic assay for lead ions in drinking water based on antibody-oriented probe and silver enhancement.  EUROPEAN FOOD RESEARCH AND TECHNOLOGY,  249  (12): (3097-3103).  [PMID:] [10.1007/s00217-023-04351-5]

2. Jin Li, Xintong Li, Yulian Wang, Qianxu Zhou, Guoqing Shi.  (2024)  Utilization of Fc-specific peptide to orientally immobilize antibodies markedly improves the analytical performance of lateral flow immunoassay strips for aflatoxin B1.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.112404]

3. Shuo Yang, Jinglong Qu, Yang Qin, Shuaibing An, Kaiqiang Huang, Yaling Wei, Jun Xi.  (2026)  Enhanced ultra-sensitive detection of peanut allergen Ara h 3 on immunochromatographic paper based on surface-enhanced Raman spectroscopy and AuNBs@PDA dual signal amplification strategy.  FOOD CHEMISTRY,      [PMID:41638166] [10.1016/j.foodchem.2026.148209]

4. Pan Cheng, Chuang Zhang, Xiupu Lu, Daiqing Peng, Pengju Zhao, Tao Mei, Shunxing Zhang, Qihao Guo, Ke Liu, Dong Wang.  (2026)  Protein A functionalized hierarchically ordered nanofibrous sponge enabling high-efficiency IgG purification.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2026.140254]