GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
Coreceptor for bacterial lipopolysaccharide.
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS).
Acts via MyD88, TIRAP and
Host species
Mouse
Specificity
Human CD14
Immunogen
Human Thymocytes followed by peripheral blood mononuclear cells.
Positive Control
Flow Cytometry: Human peripheral blood monocytes
Conjugation
Pacific Blue
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD14 Antibody (Pacific Blue) (Ab223476) - Flow Cytometry Flow cytometry analysis of CD14 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD14 Antibody (Pacific Blue) (Ab223476) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (Pacific Blue) (Ab216764).
Recombinant CD14 Antibody (Pacific Blue) (Ab223476) - Flow Cytometry Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD14 Antibody (Pacific Blue) (Ab223476) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (Pacific Blue) (Ab216764). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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