GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
ExactAb™, Validated, Recombinant, 5 μL/test ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Recombinant CD16 Antibody, clone P06F04, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD16 Antibody (AF594) - Primary antibody, specific to CD16
Synonyms
CD 16 antibody | CD 16a antibody | CD16 antibody | CD16a antibody | CD16a antigen antibody | CD16B antibody | CD16b antigen antibody | Fc fragment of IgG antibody | Fc fragment of IgG low affinity IIIa receptor (CD16) antibody | Fc fragment of IgG low aff
Specifications & Purity
ExactAb™, Validated, Recombinant, 5 μL/test
Biochemical and Physiological Mechanisms
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Host species
Mouse
Specificity
CD16
Immunogen
Human NK Cells
Positive Control
Flow Cytometry: Human peripheral blood lymphocytes
Conjugation
AF594
Excitation (nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD16 Antibody (AF594) (Ab223493) - Flow Cytometry Flow cytometry analysis of CD16 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD16 Antibody (AF594) (Ab223493) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF594) (Ab190247).
Recombinant CD16 Antibody (AF594) (Ab223493) - Flow Cytometry Flow cytometry analysis of CD16 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD16 Antibody (AF594) (Ab223493) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF594) (Ab190247). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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